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Invitrogen™ TRAIL (TNFSF10) Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMTNFSF10

Encompass

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Catalog No. EMTNFSF10



Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Mouse TRAIL (TNFSF10) ELISA quantitates Ms TRAIL in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TRAIL. Principle of the method The Mouse TRAIL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
Specifications

Specifications

Colorimetric Microplate Reader
ELISA Kit
Biotin
Cytokines & Receptors
P50592
2pg/mL to 600pg/mL
22035
RUO
2°C to 8°C
4 hr. 45 min.
Immunology
<12%
96 Tests
Plasma, Serum, Supernatant
A330042I21Rik,AI448571,APO-2L,Ly81,TL2,Tnlg6a,Trail
96 assays
This ELISA pair antibody detects mouse TRAIL. Other species not determined.
Solid-phase sandwich ELISA
HRP
2.46-600 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Mouse
A330042I21Rik, AI448571, APO-2L, Ly81, TL2, Trail
<10%
Mouse
2 pg/mL
SDS
Documents
Product Certifications
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