The Mouse TRANCE (RANKL/TNFSF11) ELISA quantitates Ms TRANCE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms TRANCE. Principle of the method The Mouse TRANCE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.RANKL (Receptor Activator of Nuclear Factor kappa-B Ligand,Trance, TNFSK11) is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Structurally, RANKL shares the structural scaffold of TNF family members but have individual receptor-ligand pairs that associate with high specificity. RANKL has been shown to self- associate as a homotrimer with four surface loops distinct from other TNF-type molecules. RANKL was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of RANKL and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate anti-apoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated that RANKL may have a role in the regulation of cell apoptosis. Targeted disruption of a gene related to RANKL in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found for RANKL.
|2°C to 8°C|
|Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL|
|1 hr. 20 min.|
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