BioVision Inc Transglutaminase Activity Assay Kit (Colorimetric): Validated using many sample types such as Cell lysate, Tissue lysate & Recombinant Transglutaminase. Detection limit: ~10 µU or 80 ng of recombinant hTG2 enzyme. 100 assay
Manufacturer: BioVision Inc K571100
Transglutaminase Activity Assay Kit (Colorimetric): Transglutaminases (EC 18.104.22.168) are calcium dependent enzymes that catalyze the post-translational modification of proteins by formation of isopeptide bonds. The transglutaminase enzyme family comprises the intracellular forms (TG1, TG3 and TG5), TG2 which is both intracellular and extracellular, TG4 which is expressed in the prostate gland, factor XIII which is expressed in blood, TG6 and TG7, whose tissue distribution is unknown and band 4.2 (lacking enzymatic activity) which is present on erythrocyte membranes. BioVision's Transglutaminase Activity Assay kit utilizes the deamidation reaction of the transglutaminase enzyme with a donor and acceptor substrate resulting in the formation of a hydroxamate product. The hydroxamate product reacts with the Stop Solution forming a purple complex that can be measured colorimetrically at 525 nm. The limit of quantification of this assay is ~10 ¿U or 80 ng of recombinant hTG2 enzyme.
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