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Invitrogen™ TSLP Human ELISA Kit Non-distribution product as customer accommodation.

Sandwich ELISA Kit



Storage Requirements 2°C to 8°C
Assay Sensitivity 3 pg/mL
Assay Range 3.28-800 pg/mL
Test Time 1 hr. 20 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
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EHTSLP Non-distribution product as customer accommodation.
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96 Tests
Pack for $486.00
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The Human TSLP ELISA quantitates Hu TSLP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TSLP. Principle of the method The Human TSLP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.


2°C to 8°C
3.28-800 pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
3 pg/mL
1 hr. 20 min.
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