Tubulin-α Ab-2 Mouse Monoclonal Antibody, Epredia™

Provide accurate, reproducible results with the Epredia™ Tubulin-α Ab-2, Mouse Monoclonal Antibody.

Supplier:  Epredia MS581P1

Catalog No. MS581P1

Description

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: α and Β. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Host Species: Mouse

Clone: DM1A

Isotype: IgG1/κ

Species Reactivity: Human, Cow, Pig, Rat, Mouse, Guinea Pig, Gerbil, Frog and Chicken. Others not known.

Epitope: aa 426-450

Immunogen: Native chick brain microtubules

Molecular Weight: 57kDa

Positive Control: Ls174T, MAD109 cells; skin or lung

Cellular Localization: Cytoplasmic Ab-2 causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunofluorescence
• Immunoprecipitation (Native and denatured)
• Electron Microscopy

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: Tubulin, alpha Ab-2
• Classification: Monoclonal
• Concentration: 200μg/mL
• Host Species: Mouse
• Quantity: 500 μL
• Research Discipline: Cytoskeletal Protein
• Target Species: Bovine, Chicken, Frog, Gerbil, Guinea Pig, Human, Mouse, Porcine, Rat
• Isotype: IgG2a κ

• Applications: Immunoassay, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: DM1A
• Conjugate: Unconjugated
• Immunogen: Native chick brain microtubules
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Concentrated