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alpha Tubulin Mouse anti-Canine, Human, Mouse, Non-human primate, Porcine, Rat, Alexa Fluor® 488, Clone: DM1A, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 53450282

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Catalog No. 50-167-46


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Description

Description

Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430). Applications Reported: This DM1A antibody has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: This DM1A antibody has been tested by immunocytochemistry of adherent cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predomitly found in morphologically differentiated neurologic cells.
Specifications

Specifications

alpha Tubulin
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P68370, P02550, P68369, Q71U36
FLJ30169, H2 ALPHA, Testis specific alpha tubulin, TUBA1, Tubulin alpha
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Human, Mouse, Non-human primate, Porcine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
DM1A
Alexa Fluor™ 488
TUBA1A
Liquid
Tuba1a
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
100135051, 22142, 64158, 7846
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