Invitrogen™ TWEAK (TNFSF12) Human ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EHTNFSF12	Invitrogen™ EHTNFSF12	96 Tests
EHTNFSF12X5	Invitrogen™ EHTNFSF12X5	5 x 96 Tests

Description

The Human TWEAK (TNFSF12) ELISA quantitates Hu TWEAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TWEAK. Principle of the method The Human TWEAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Specifications

• Accession Number: O43508
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 8742
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Angiogenesis
• Synonym: UNQ181/PRO207, APO3L, DR3LG, TWEAK
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 40 pg/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: APO3L,DR3LG,TNLG4A,TWEAK
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.