Invitrogen™ UBE2B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521316

Catalog No. PIPA521316

Description

Recommended positive controls: mouse brain, 3xFlag-human UBE2B-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Specifications

• Antigen: UBE2B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ube2b
• Gene Alias: 2610301N02Rik; E2 protein; E2 ubiquitin-conjugating enzyme B; E2(14k); E214K; E2-14k; E2-17kDa; HHR6B; HR6B; mHR6B; RAD6 homolog B; Rad6b; UBC2; Ube2b; Ubiquitin carrier protein B; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2 B; ubiquitin conjugating enzyme E2B; ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2(14k); ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2B (RAD6 homolog, S. cerevisiae); ubiquitin-conjugating enzyme E2B, RAD6 homolog; ubiquitin-conjugating enzyme E2B, RAD6 homology; ubiquitin-protein ligase B
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22210, 7320
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 2.72 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P63146, P63147
• Gene Symbols: Ube2b
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 182 of UBE2B.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG