Ubiquitin Recombinant Rabbit Monoclonal Antibody (10H4L21), Invitrogen™
Rabbit Recombinant Monoclonal Antibody
Supplier: Thermo Scientific 701339
701339 was successfully used to detect the ubiquitination of GLUT1 in transfected in HeLa cells. This antibody is predicted to react with bovine, chimpanzee, equine, non-human primate, rabbit, ovine and porcine based on sequence homology. Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain. Recombit rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombit rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to posttranslation modifications. The degradation of cellular regulatory proteins by the ubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. First reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.
|PBS with 0.09% sodium azide|
|Peptide corresponding to amino acids 32–58 of human ubiquitin|
|ChIP assay, Immunocytochemistry, Immunofluorescence, Western Blot|
|60S ribosomal protein L40, HUBCEP52, RPL40, Ubiquitin 52 amino acid fusion protein, Ubiquitin A52 residue ribosomal protein fusion product 1, UBB, UBC|
|Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
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