R&D Systems™ Universal Chemiluminescent PARP Assay - Histone Coated Wells
Chemiluminescent assay kit for candidate inhibitor screening and determination of IC50 values of PARP inhibitors.
Manufacturer: R&D Systems™ 4676096K
HT Universal 96-well PARP Assay Kits measure the incorporation of biotinylated poly(ADP-ribose) onto histone proteins in a strip well format. This assay is ideal for the screening of PARP inhibitors and determining IC<sub>50</sub> values. Important features of the assay include: 1) chemiluminescent, non-radioactive format; 2) higher throughput 96 test size; and 3) sensitivity down to 0.01 Units of PARP per well.
- PARP-HSA, 10 U/μL 50 μL
- 20X PARP Buffer (2 vials) 2 x 500 μL
- 10X PARP Cocktail (300 µlL)
- Histone-Coated White Strip Wells (96 Wells)
- 200 mM 3-Aminobenzamide (60 μL)
- 10X Strep-Diluent (2 mL)
- Strep-HRP (30 μL)
- PeroxyGlow™ A (6 mL)
- PeroxyGlow™ B (6 mL)
- 10X Activated DNA (300 μL)
|Store the unopened product at -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.|
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