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Invitrogen™ Uromodulin (UMOD) Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHUMOD

Encompass

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Catalog No. EHUMOD


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Description

Description

The Human Uromodulin (UMOD) ELISA quantitates Hu UMOD in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu UMOD. Principle of the method The Human UMOD solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.
Specifications

Specifications

ELISA Kit
96 assays
Other
Biotin
P07911
40pg/mL to 10,000pg/mL
7369
RUO
2°C to 8°C
4 hr. 45 min.
Immunology
<12%
96 Tests
Plasma, Serum, Supernatant
ADMCKD2,FJHN,HNFJ,HNFJ1,MCKD2,THGP,THP
This ELISA pair antibody detects human Uromodulin. Other species not determined.
Colorimetric Microplate Reader
Solid-phase sandwich ELISA
HRP
40.96-10,000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
ADMCKD2, FJHN, HNFJ, HNFJ1, MCKD2, THGP, THP
<10%
Human
40 pg/mL
SDS
Documents
Product Certifications
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