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Vγ9 TCR Mouse anti-Human, Brilliant Violet 605, Clone: B3, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 744036

Catalog No. BDB744036


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Description

Description

The B3 monoclonal antibody specifically recognizes the human variableγ9 subunit (Vγ9) ofγ/δ T- cell receptor (TCR). Theγ/δTCR is composed of two disulfide-linked glycoproteins. The gamma chain is approximately 45-60 kD and is detected on a small subset peripheral blood T cells and thymocytes. The majority of normal peripheral bloodγ/δT cells express a Vγ9+/Vδ2+ phenotype. The reason for this selection in the T-cell repertoire is not well understood.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
Specifications

Specifications

Vγ9 TCR
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Vgamma9 TCR, V2, TRGC1, TCRGV9, TRGV9
Mouse IgG1, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
B3
Brilliant Violet 605
Affinity Purified
Murine
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Human
SDS
Documents
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