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TCR V alpha 24 J alpha 18 Mouse anti-Human, PE, Clone: 6B11, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12580642

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Catalog No. 50-112-2333


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Description

Description

Description: The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the V alpha24 and J alpha18 of the TCR alpha locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine V alpha 14 J alpha 18. This TCR alpha chain pairs with a restricted set of rearranged TCR beta chains, with V beta 11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-presented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFN gamma. Although iNKT cells have NK-like cytolytic activity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells. Applications Reported: This 6B11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6B11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
Specifications

Specifications

TCR V alpha 24 J alpha 18
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
TCRV alpha 24 J alpha 18; TCRV alpha24 J alpha18; TCR V alpha24 J alpha18; Valpha24; Va24
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
Human
Flow Cytometry
6B11
PE
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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