TCR V beta 11 Monoclonal Antibody (RR3-15), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 17582782

Catalog No. 50-112-3144

Description

Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity. The RR3-15 antibody has been reported to activate V beta 11+ T cells. Applications Reported: This RR3-15 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

TCR V beta 11 Monoclonal antibody specifically detects TCR V beta 11 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TCR V beta 11
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: RR3-15
• Conjugate: APC
• Gene Alias: TCR V beta11; TCRV beta 11; TCRV beta11; Vb11; Vbeta11
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.