Novus Biologicals™ VE-Cadherin Antibody Pair [HRP]

Monoclonal Antibody Pair

Catalog Number	Mfr. No.	Quantity
NB009965	Novus Biologicals™ NBP27946315PLATES	15 Plates

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human VE-Cadherin.

Specifications

• Assay Range: 93.75 pg/mL
• Assay: Sandwich ELISA
• Product Type: Antibody Pairs
• Target Species: Human
• Gene Alias: 7B4, 7B4 antigen, cadherin 5, type 2 (vascular endothelium), cadherin 5, type 2, VE-cadherin (vascular epithelium), cadherin-5, CD144, CD144 antigen, endothelial-specific cadherin, FLJ17376, Vascular endothelial cadherin, VE-cadherin
• Gene Symbol: CDH5
• Kit Contents:
• Detection Antibody: 0.2 mg/mL of rabbit anti-Human VE-Cadherin monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Capture Antibody: 0.5 mg/mL of mouse anti-Human VE-Cadherin monoclonal antibody (in PBS, pH 7.4).
• Standard: Each vial contains 400 ng of recombinant Human VE-Cadherin.
• Research Discipline: Cancer, Cell Cycle and Replication, Cellular Markers, Extracellular Matrix, Phospho Specific, Plasma Membrane Markers, Signal Transduction
• Target: VE-Cadherin

• Assay Sensitivity: 93.75-6000 pg/mL
• Conjugate: HRP
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human VE-Cadherin.
• For Use With (Application): Sandwich ELISA
• Gene ID (Entrez): 1003
• Host Species: Rabbit/Mouse
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 6000 pg/mL is recommended.