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Vimentin Mouse anti-Canine, Chicken, Human, Rat, Clone: V9, eBioscience™ Invitrogen™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

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Catalog No. 14-989-795


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Description

Description

The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils. This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin. Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P). Applications Tested: This V9 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized SK-N-SH or C6 cell lines and by western blot on cell lysates prepared from HeLa cells. This antibody can be used at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Vimentin Monoclonal antibody specifically detects Vimentin in Canine, Chicken, Human, Rat samples. It is validated for Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Specifications

Specifications

Vimentin
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
V9
Unconjugated
Vim
cb28; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; Vim; VIME; Vimentin
IgG1, kappa
10 mg
4° C
Primary
420519, 477991, 7431, 81818
Liquid
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P08670, P09654, P31000
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Chicken, Human, Rat
SDS
Documents
Product Certifications
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