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Vimentin Mouse anti-Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse, Rat, Alexa Fluor™ 750, Clone: VM452, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus Biologicals NBP233060AF750

Catalog No. NP233060A70

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Vimentin Monoclonal antibody specifically detects Vimentin in Human,Porcine,Bovine,Canine,Chicken,Feline,Goat,Mouse,Rat samples. It is validated for Western Blot,ELISA.


Western Blot, ELISA, Knockout Validated
FLJ36605, vimentin
Store at 4°C in the dark.
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP′s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
ELISA, Immunoassay, Western Blot
Alexa Fluor™ 750
50 mM sodium borate with 0.05% sodium azide
A recominant protein fragment specific to Vimentin was used as the immunogen for the VM452 antibody.
0.1 mL
Bovine, Canine, Chicken, Feline, Goat, Human, Mouse, Porcine, Rat
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