VISTA Mouse anti-Human, PE, Clone: B7H5DS8, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: eBioscience 12108841
Description: This B7H5DS8 monoclonal antibody recognizes human VISTA, also known as B7-H5 or PD-1H. This clone has been tested and shown not to cross-react with human B7-H4, B7-H6 and B7-H7. This B7H5DS8 antibody will work in flow cytometry only on live and not paraformaldehyde-fixed cells. Applications Reported: This B7H5DS8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B7H5DS8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserVISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-H1, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans VISTA is expressed predomitly on hematopoietic cells with the highest densities on CD11b+ myeloid cells, and somewhat lower levels on CD4 and CD8 T cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. Mice deficient in VISTA are viable and have elevated frequencies of activated T cells but do not spontaneously develop autoimmune disorders.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|B7-H5, PD-H1, Gi24, Dies1, SISP1, C10orf54, PD-1H|
|4° C, store in dark, DO NOT FREEZE!|
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