Description: This B7H5DS8 monoclonal antibody recognizes human VISTA, also known as B7-H5 or PD-1H. This clone has been tested and shown not to cross-react with human B7-H4, B7-H6 and B7-H7. This B7H5DS8 antibody will work in flow cytometry only on live and not paraformaldehyde-fixed cells. Applications Reported: This B7H5DS8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B7H5DS8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserVISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-H1, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans VISTA is expressed predomitly on hematopoietic cells with the highest densities on CD11b+ myeloid cells, and somewhat lower levels on CD4 and CD8 T cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. Mice deficient in VISTA are viable and have elevated frequencies of activated T cells but do not spontaneously develop autoimmune disorders.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|B7-H5, PD-H1, Gi24, Dies1, SISP1, C10orf54, PD-1H|
|4° C, store in dark, DO NOT FREEZE!|
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok