Description: The monoclonal antibody 3G6 recognizes human, mouse, and rat ZEB1. ZEB1, or zinc finger E-box binding homeobox 1, is also known as Transcription Factor 8 (TCF8), Negative Regulator of IL2, and AREB6. ZEB1 acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 also regulates expression of the genes E-cadherin, BCL6, ATP1A1, and RCOR1 through binding to multiple enhancer boxes (E-boxes). ZEB1 promotes epithelial mesenchymal transition (EMT) and invasion of various types of cancers, such as colorectal, breast, pancreatic, lung, and gallbladder. In normal tissues, ZEB1 is expressed in tissues undergoing differentiation such as T cell and skeletal differentiation. Mutations in ZEB1 have been associated with corneal dystrophy. Applications Reported: This 3G6 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 3G6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
|Human, Mouse, Rat|
|Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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