Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Zenon™ Mouse IgG1 Labeling Kit

Simplify your cell staining applications with Invitrogen Zenon™ Mouse Antibody IgG1 Antibody Labeling Kits.

$391.00 - $411.00


Products 11
Catalog Number Mfr. No. No. of Reactions Label or Dye Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Label or Dye Price Quantity & Availability  
Z25000 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25000
50 Alexa Fluor™ 350 Each for $391.00
Add to cart
 
Z25002 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25002
50 Alexa Fluor™ 488 Each for $407.00
Add to cart
 
Z25004 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25004
50 Alexa Fluor™ 546 Each for $395.00
Add to cart
 
Z25005 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25005
50 Alexa Fluor™ 555 Each for $402.00
Add to cart
 
Z25006 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25006
50 Alexa Fluor™ 568 Each for $403.00
Add to cart
 
Z25007 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25007
50 Alexa Fluor™ 594 Each for $402.00
Add to cart
 
Z25008 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25008
50 Alexa Fluor™ 647 Each for $411.00
Add to cart
 
Z25052 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25052
50 Biotin-XX Each for $393.00
Add to cart
 
Z25054 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25054
25 HRP (Horseradish Peroxidase) Each for $394.00
Add to cart
 
Z25060 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25060
3x10 Alexa Fluor™ 488, 555, 647 Each for $393.00
Add to cart
 
Z25090 Non-distribution product as customer accommodation.
View Documents
Molecular Probes™
Z25090
50 Alexa Fluor™ 488 Each for $393.00
Add to cart
 
Description

Description

Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.
Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:

· Labeled antibodies are typically ready to use in 10 minutes
· Requires only 1–20 µg primary antibody
· Simple—no purification required
· Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase
· Multiplex with other mouse monoclonal antibodies simultaneously
· Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.
Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.
Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.
Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.
Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.
Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit