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Restriction Enzymes

Value of Choosing Fisher Bioreagents™ OPTIZYME™ Restriction Enzymes

Browse All Restriction Enzymes & Buffers

Purity: OPTIZYME restriction enzymes are produced using high-yielding expression vectors, resulting in a higher level of purity and lower processing time than native restriction enzymes.

Consistency: OPTIZYME restriction enzymes are manufactured in class D clean rooms using recombinant production techniques to yield large lot sizes. The combination of leading production technology with ISO9001 quality and ISO14001 environmental management systems ensures high digestion and ligation efficiency, as well as batch-to-batch reproducibility.

Value Pricing: The use of high-yielding expression vectors and bacterial strains enables Fisher BioReagents to offer recombinant enzymes with the lowest price/unit charges, allowing our customers to benefit from both substantial savings and improved consistency and quality.

FisherChemical_BuildingProd

OPTIZYME DNA/RNA Modifying Enzymes

OPTIZYME DNA/RNA Modifying Enzymes are manufactured using high yielding expression vectors in class D clean rooms under strict ISO9001 quality and ISO14001 environmental management systems. By combining leading recombinant production technology with value pricing and batch-to-batch consistency, OPTIZYME customers benefit from both substantial savings and improved quality and consistency on modifying enzymes used for:

  • DNA cloning
  • In vitro transcription
  • cDNA synthesis

Key features:

  • Quality assurance tested for functional purity and activity
  • Nuclease-free
  • Value pricing
  More
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OPTIZYME Restriction Enzymes

Genomics applications, such as gene cloning and DNA genotyping, require high-quality restriction enzymes. The Fisher BioReagents OPTIZYME line of restriction enzymes offers customers an outstanding combination of quality, performance and low prices.

Outstanding performance and convenience:

  • Economy: Competitively priced
  • Quality: Outstanding quality and consistency
  • Selection: A broad range of the most commonly used restriction enzymes
  • Packaging: Product specifications are presented in a user-friendly and convenient format
  More

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BioReagent Buffers

Buffers for Every Budget and Scale

  • Economical powders come in various package sizes
  • Concentrated stock solutions provide convenience in one step of easy dilution
  • Ready-to-use solutions offer the biggest time savings of all

Browse All BioReagent Buffers


fisher-bioreagents-cell-pure-btl

Fisher Bioreagents™ CellPURE™ Biological Buffers

The purpose of a buffer in a biological system is to maintain intracellular and extracellular pH within the physiological range and resist changes in pH due to the presence of internal and external influences. CellPURE Biological Buffers from Fisher BioReagents are ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Norman Good and co-workers in 1966, these organic buffers have pKa values at or near physiological pH and minimal interference with biological processes. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays and other biochemical applications.

(Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477)

  • Optimized for cell/tissue culture work
  • Ultrapure zwitterionic biological buffers
  • Wide applicability to molecular biology and biochemical studies
  • Manufactured under strict quality control guidelines to ensure performance and reliability

Advantages

  • Cell culture tested
  • Analyzed for the absence of nucleases and proteases
  • Tested for endotoxin and bioburden levels
  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Minimal permeability to biological membranes

Applications

  • Tissue culture media and maintenance of cell lines
  • Enzyme assays
  • Electrophoresis of RNA
  • Transfection of mammalian cells
  More

Properties of CellPURE Biological Buffers

Description Formula Molecular Weight pKa @ 25º C Useful pH Range

Description

Formula
C₈H₁₉NO₅
Molecular Weight
209.24
pKa @ 25º C
6.5
Useful pH Range
5.8 to 7.2

Description

Formula
C₁₁H₂₆N₂O₆
Molecular Weight
282.33
pKa @ 25º C
6.8†
Useful pH Range
6.3 to 9.5

Description

BES
Formula
C₆H₁₅NO₅₅
Molecular Weight
213.25
pKa @ 25º C
7.1
Useful pH Range
6.4 to 7.8

Description

Formula
C₇H₁₅NO₄S
Molecular Weight
209.26
pKa @ 25º C
7.2
Useful pH Range
6.5 to 7.9

Description

Formula
C₇H₁₄NNaO₄S
Molecular Weight
231.25
pKa @ 25º C
7.2
Useful pH Range
6.5 to 7.9

Description

Formula
C₈H₁₈N₂O₄S
Molecular Weight
238.3
pKa @ 25º C
7.5
Useful pH Range
6.8 to 8.2

Description

Formula
C₈H₁₇N₂NaO₂S
Molecular Weight
260.29
pKa @ 25º C
7.5
Useful pH Range
6.8 to 8.2

Description

TES
Formula
C₆H₁₅NO₈S
Molecular Weight
229.25
pKa @ 25º C
7.5
Useful pH Range
6.8 to 8.2

Technical Tip

The Water Makes a Difference. CellPURE Biological Buffers are of the highest quality (purity ≥ 99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk for contamination of buffer solutions. Fisher BioReagents provides several grades of high-purity water suitable for the preparation of high-quality buffer solutions.

fisher-bioreagents-just-pure-btl

JustPURE "Good" Buffers

Zwitterionic buffers possessing both positive and negative charges were described in 1966 by Good and co-workers as being suitable for work with biological molecules. Popularly known today as “Good” buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate and bicarbonate.

“Good” buffers approach the “ideal” buffer state by having pKa values at or near physiological pH and minimal interference with biological processes. JustPURE “Good” buffers from Fisher BioReagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content.

(Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477)

  • Ultrapure zwitterionic buffers
  • Optimized for research in cellular and molecular biology
  • Low interference with biological reactions

Advantages

  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Chemically and enzymatically stable
  • Minimal permeability to biological membranes
  • Limited effect on biochemical reactions
  • Minimal absorption in spectral range 240 to 700nm

Applications

  • High efficiency transfection of mammalian cells
  • Gel electrophoresis of RNA
  • Protein isolation applications
  • Cell cultures and enzyme assays
  • Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE
  More

Product Description CAS # Formula Molecular Weight pKa @ 25º C Useful pH Range

Product Description

CAS #
4432-31-9
Formula
C₆H₁₃NO₄SxH₂O
Molecular Weight
195.24 (anhydrous)
pKa @ 25º C
6.1
Useful pH Range
5.5 - 6.7

Product Description

CAS #
7365-82-4
Formula
C₄H₁₀N₂O₄S
Molecular Weight
182.2
pKa @ 25º C
6.8
Useful pH Range
6.1 - 7.5

Product Description

CAS #
5625-37-6
Formula
C₈H₁₈N₂O₆S₂
Molecular Weight
302.37
pKa @ 25º C
6.8
Useful pH Range
6.1 - 7.5

Product Description

CAS #
64431-96-5
Formula
C₁₁H₂₆N₂O₆
Molecular Weight
282.33
pKa @ 25º C
6.8a
Useful pH Range
6.3 - 9.5

Product Description

CAS #
71119-22-7
Formula
C₇H₁₄NNaO₄S
Molecular Weight
231.25
pKa @ 25º C
7.2
Useful pH Range
6.5 - 7.9

Product Description

CAS #
1132-61-2
Formula
C7H15NO4S
Molecular Weight
209.26
pKa @ 25º C
7.2
Useful pH Range
6.5 - 7.9

Product Description

CAS #
7365-45-9
Formula
C₈H₁₈N₂O₄S
Molecular Weight
238.3
pKa @ 25º C
7.5
Useful pH Range
6.8 - 8.2

Product Description

CAS #
75277-39-3
Formula
C₈H₁₇N₂NaO₄S
Molecular Weight
260.29
pKa @ 25º C
7.5
Useful pH Range
6.8 - 8.2

Product Description

CAS #
16052-06-5
Formula
C₉H₂ON₂O₄S
Molecular Weight
252.33
pKa @ 25º C
8
Useful pH Range
7.3 - 8.7

Product Description

CAS #
- -
Formula
C₆H₁₃NO₅
Molecular Weight
179.17
pKa @ 25º C
8.1
Useful pH Range
7.4 - 8.8

Product Description

CAS #
556-50-3
Formula
C₄H₈N₂O₃
Molecular Weight
132.12
pKa @ 25º C
8.2
Useful pH Range
7.5 - 8.9

Product Description

CAS #
29915-38-6
Formula
C₇H₁₇NO₈S
Molecular Weight
243.28
pKa @ 25º C
8.4
Useful pH Range
7.7 - 9.1

Product Description

CAS #
103-47-9
Formula
C₈H₁₇NO₃S
Molecular Weight
207.29
pKa @ 25º C
9.3
Useful pH Range
8.6 - 10.0

Product Description

CAS #
1135-40-6
Formula
C₉H₁₉NO₃S
Molecular Weight
221.32
pKa @ 25º C
10.4
Useful pH Range
9.7 - 11.1

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Buffers for Nucleic Acid Electrophoresis

Buffers for Hybridization of Nucleic Acids

Antibiotics and Antimycotics

Optimized for Cell Culture

fisher-bioreagents-grp-antibiotics

In many life science laboratories, the in vitro culturing of bacterial, plant and animal cells is a routine task. High-quality antibiotics and antimycotics from Fisher BioReagents can be used to ensure successful growth of cells by eliminating unwanted bacterial strains and fungi, while maintaining the health and vitality of the desired cells.

Browse All Antibiotics

Advantages

  • High quality - antibiotics and antimycotics meet rigorous quality control tests, including verification of potency, purity and solubility using microbiological and chromatographic methods
  • Built for selection - excellent choice of antibiotics for selection of antibiotic resistance genes in both bacterial and mammalian cells
  • High purity and stability - ultrapure antibiotics ensure successful growth of target cells and reproducible results
  • Maximum convenience - antibiotics and antimycotics come in both sterile powder and liquid forms

Applications

  • Plant cell culture
  • Mammalian cell culture
  • Tissue culture
  • Genetic marker selection
  More

Antibiotic Modes of Action

Catalog No. Product Description Molecular Weight Mode of Action Susceptible Organisms Form Size Packaging

Catalog No.

Product Description
Bacitracin
Molecular Weight
1422.7
Mode of Action
Inhibitor of protein disulfide isomerase. Interferes with building blocks of peptidoglycan bacterial cell wall.
Susceptible Organisms
Gram+
Form
Dry
Size
1g
Packaging
Amber glass bottle

Catalog No.

Product Description
Cefotaxime Sodium Salt
Molecular Weight
477.5
Mode of Action
Inhibitor of bacterial cell wall synthesis. Eliminates Agrobacterium species after inoculation.
Susceptible Organisms
Gram -
Form
Dry
Size
1g
Packaging
Amber glass bottle

Catalog No.

Product Description
Hygromycin B
Molecular Weight
527.5
Mode of Action
Inhibits protein synthesis
Susceptible Organisms
Prokaryotes and higher eukaryotes
Form
Liquid
Size
1mu
Packaging
Crimp top on glass bottle

Catalog No.

Product Description
Nystatin
Molecular Weight
926.1
Mode of Action
Alters membrane permeability and allows potassium ion leakage.
Susceptible Organisms
Yeasts and molds
Form
Dry
Size
5g
Packaging
Amber glass bottle

Catalog No.

Product Description
Paromomycin Sulfate
Molecular Weight
615.6
Mode of Action
Inhibits protein synthesis
Susceptible Organisms
Gram+ and Gram -
Form
Dry
Size
1g
Packaging
Amber glass bottle

Catalog No.

Product Description
Penicillin G Sodium Salt
Molecular Weight
356.4
Mode of Action
Interferes with peptidoglycan synthesis in bacterial cell walls.
Susceptible Organisms
Gram+
Form
Dry
Size
5g
Packaging
Amber glass bottle

Catalog No.

Product Description
Penicillin/Streptomycin Mixture
Molecular Weight
*
Mode of Action
Interferes with synthesis of bacterial cell wall. Inhibits protein synthesis; binds to 30S ribosomal subunit.
Susceptible Organisms
Gram+ and Gram -
Form
Liquid
Size
50mL
Packaging
Clear plastic bottle

Catalog No.

Product Description
Penicillin/Streptomycin/Glutamine Mixture
Molecular Weight
*
Mode of Action
Interferes with synthesis of bacterial cell wall. Inhibits protein synthesis. Interferes with bacterial cell division.
Susceptible Organisms
Gram+ and Gram -
Form
Liquid
Size
50mL
Packaging
Clear plastic bottle

Catalog No.

Product Description
Penicillin/Streptomycin/Neomycin Mixture
Molecular Weight
*
Mode of Action
Interferes with synthesis of bacterial cell wall. Inhibits protein synthesis.
Susceptible Organisms
Gram+ and Gram -
Form
Liquid
Size
50mL
Packaging
Clear plastic bottle

Catalog No.

Product Description
Phleomycin
Molecular Weight
1526.5
Mode of Action
Disrupts the integrity of DNA, and blocks S-phase entry in the cell cycle.
Susceptible Organisms
Bacteria, yeast, fungi, plant and animal cells
Form
Liquid
Size
100mg
Packaging
Clear plastic bottle

Catalog No.

Product Description
Polymixin B Sulfate
Molecular Weight
1385.61
Mode of Action
Interferes with cytoplasmic membrane.
Susceptible Organisms
Gram -
Form
Dry
Size
1g
Packaging
Amber glass bottle

Catalog No.

Product Description
Puromycin Dihydrochloride
Molecular Weight
544.4
Mode of Action
Inhibits protein synthesis.
Susceptible Organisms
Gram+ and various animal and insect cells
Form
Dry
Size
100mg
Packaging
Clear plastic bottle

Catalog No.

Product Description
Rapamycin
Molecular Weight
914.2
Mode of Action
Possesses immunosuppressive properties. Blocks a protein that is involved in cell division. A type of serine/threonine kinase inhibitor.
Susceptible Organisms
Yeasts and filamentous fungi, antineoplastic properties
Form
Dry
Size
1mg
Packaging
Clear glass bottle

Catalog No.

Product Description
Spectinomycin Dihydrochloride Pentahydrate
Molecular Weight
495.3
Mode of Action
Inhibits protein synthesis
Susceptible Organisms
Gram+ and Gram -
Form
Dry
Size
1g
Packaging
Amber glass bottle

Catalog No.

Product Description
Vancomycin Hydrochloride
Molecular Weight
1485.7
Mode of Action
Inhibits formation of peptidoglycan polymers of bacterial cell wall.
Susceptible Organisms
Gram+
Form
Dry
Size
1g
Packaging
Amber glass bottle

Selective Antibiotics Used in Transformation Studies

Catalog No. Product Description Formula Antibiotic Resistance Genes

Catalog No.

Product Description
Hygromycin B
Formula
C₂₀H₃₇N₃O₁₃
Antibiotic Resistance Genes
The aph4 gene (also known as hph or hyg) encodes an enzyme, hygromycin phosphotransferase, which confers resistance to hygromycin B. This antibiotic is particularly useful as a selective agent for the incorporation of aph4 in plant tissue.

Catalog No.

Product Description
Phleomycin
Formula
C₅₅H₈₅O₂₁N₂₀S₂CuHCl
Antibiotic Resistance Genes
Phleomycin resistance is conferred by the Sh ble gene from Streptoalloteichus hindustanus, which encodes a protein that binds to phleomycin, inhibiting its DNA cleavage activity. Useful in transformation studies involving plant and mammalian cells.

Catalog No.

Product Description
Puromycin Dihydrochloride
Formula
C₂₂H₂₉N₇O₅₂HCl
Antibiotic Resistance Genes
Selection agent for cells transformed with pac gene. Used for stable transfection of DNA into mammalian cells.

Catalog No.

Product Description
Spectinomycin Dihydrochloride Pentahydrate
Formula
C₁₄H₂₄N₂O₇Â₂HCl₅H₂O
Antibiotic Resistance Genes
Resistance to spectinomycin may develop by mutations in rpsL gene that prevent this antibiotic from binding to the 30S ribosomal subunit and inhibiting proper translation; streptomycin resistance is recessive.

Additional Antibiotics Available from Fisher BioReagents

Catalog No Product Description Physical Form

Catalog No

Product Description
Actinomycin-D
Physical Form
Red Crystals

Catalog No

Product Description
Amikacin
Physical Form
White Crystalline Powder

Catalog No

Product Description
Amphotericin B
Physical Form
Yellow Solution

Catalog No

Product Description
Amphotericin B
Physical Form
Yellow Powder

Catalog No

Product Description
Ampicillin Sodium Salt
Physical Form
Crystalline Powder

Catalog No

Product Description
Ampicillin Trihydrate
Physical Form
Off-white Powder

Catalog No

Product Description
Blasticidin S Hydrochloride
Physical Form
White Powder

Catalog No

Product Description
Chloramphenicol
Physical Form
White Powder

Catalog No

Product Description
Doxorubicin Hydrochloride
Physical Form
Reddish-orange Powder

Catalog No

Product Description
Doxycycline Hydrochloride
Physical Form
Yellow Powder

Catalog No

Product Description
Erythromycin
Physical Form
White Crystalline Powder

Catalog No

Product Description
G418 Sulfate
Physical Form
White to Off-white Powder

Catalog No

Product Description
Gentamycin Sulfate
Physical Form
White Powder

Catalog No

Product Description
Ionomycin Calcium Salt
Physical Form
*

Catalog No

Product Description
Kanamycin Sulfate
Physical Form
White Powder

Catalog No

Product Description
Ketoconazole
Physical Form
White Powder

Catalog No

Product Description
Miconazole
Physical Form
*

Catalog No

Product Description
Mitomycin C
Physical Form
Blue Powder

Catalog No

Product Description
Neomycin Sulfate
Physical Form
White Powder

Catalog No

Product Description
Penicillin-G Potassium Salt
Physical Form
White to Off-white Powder

Catalog No

Product Description
Rifampicin
Physical Form
Bright Orange Powder

Catalog No

Product Description
Staurosporine
Physical Form
*

Catalog No

Product Description
Streptomycin Sulfate
Physical Form
White to Light Yellow Powder

Catalog No

Product Description
Tetracycline Hydrochloride
Physical Form
Yellow Powder
Water
fisher-chemical-water-prod

Pre-packed, high-purity water for use in applications for the pharmaceutical, biotechnology, clinical, environmental and food safety industries.

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