General Western Blot Reagents
General Western Blot Reagents
Thermo Scientific™ Pierce™ Western Blot Signal Enhancer
Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
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MilliporeSigma™ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature
Promotions are available
MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent
Used for mounting cells on slides for detection of cell-surface antigens.
MilliporeSigma™ Calbiochem™ ImmunoPen™
Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent
Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane
Promotions are available
MilliporeSigma™ Calbiochem™ SignalBoost™ Immunoreaction Enhancer Kit
Designed to enhance desired signal in assays such as immunoblotting, dot blotting, ELISA.
MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma
Ready to use blocking reagent for western blotting immunoassays.
MP Biomedicals™ 3,3',5,5'-Tetramethylbenzidine Liquid Substrate, MP Biomedicals™
Provides consistent performance and sensitivity to deliver reproducible results. 3,3',5,5'-Tetramethylbenzidine Liquid Substrate, MP Biomedicals (TMB) is a convenient, nontoxic and ready to use stable chromogenic substrate for peroxidase (i.e. HRP) and ps
Promega ECL Western Blotting Substrate
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
Promotions are available
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Invitrogen™ Novex™ Reversible Membrane Protein Stain Kit
Used for determining protein transfer efficiency after blotting
Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 0.2 μm, 8.3 x 7.3 cm
Ideal for blotting proteins and nucleic acids
Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)
Pre-cut and pre-assembled and ready for protein blotting/transfer
Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 0.45 μm, 8.3 x 7.3 cm
Ideal for blotting proteins and nucleic acids