Cell Culture Media

Cell Culture Media




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Gibco™ RPMI 1640 Medium (ATCC modification)
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.


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Gibco™ DMEM, high glucose
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.


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Glucose Concentration | 4500 mg/L |
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Cell Line | HeLa, 293, Cos-7, and PC-12 |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,Glutamine,Phenol Red |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Serum Level | Standard Serum Supplementation |
Gibco™ Human Plasma-Like Medium (HPLM)
Gibco Human Plasma-like Medium (HPLM) is designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma.


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With Additives | Glutamine,Phenol Red |
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Product Type | Human Plasma-Like Medium |
Form | Liquid |
Without Additives | No HEPES |
Culture Type | Mammalian Cell Culture |
Shelf Life | 12 Months |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Serum Level | Standard Serum Supplementation |
Cell Type | Mammalian Cells |
Gibco™ Opti-MEM™ I Reduced Serum Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.


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Shipping Condition | Room Temperature |
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Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
Content And Storage | Store at 2–8°C and protect from light. |
Form | Liquid |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine,Phenol Red |
Product Type | Opti-MEM I |
Classification | Human Origin |
Shelf Life | 18 Months From Date of Manufacture |
Product Line | Gibco™, Opti-MEM™ |
Gibco™ Medium 254
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Medium 254


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Gibco™ IMDM, GlutaMAX™ Supplement
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IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.


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Shipping Condition | Room Temperature |
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Cell Line | Jurkat, COS-7, and macrophage cells |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No α-Thioglycerol,No 2-Mercaptoethanol |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,GlutaMAX,HEPES,Phenol Red,Sodium Pyruvate |
Product Type | IMDM (Iscove's Modified Dulbecco's Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™, GlutaMAX™ |
Gibco™ DMEM, powder, high glucose
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.


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Form | Powder |
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Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Gibco™ Williams' E Medium, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

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Gibco™ Synth-a-Freeze™ Cryopreservation Medium
Synth-a-Freeze™ Cryopreservation Medium

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Gibco™ EpiLife™ Medium, with 60 μM calcium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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EpiLife™ Medium, with 60 μM calcium

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Gibco™ DMEM, powder, high glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Cell Line | HeLa, 293, Cos-7, and PC-12 |
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
Form | Powder |
Without Additives | No HEPES,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
With Additives | High Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Classification | Animal Origin-free |
Shelf Life | 36 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ Expi293™ Expression Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Expi293™ Expression Medium designed for growth, transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.


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Product Type | Expression Medium |
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Form | Liquid |
Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
Product Line | Expi293™ |
Gibco™ ExpiCHO™ Expression Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells.


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Shipping Condition | Room Temperature |
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Cell Line | ExpiCHO-S™, CHO-S, SFM Adapted |
Content And Storage | Storage conditions: in the dark at 2-8°C Shipping conditions: ambient Shelf life: 12 months from date of manufacture |
With Additives | GlutaMAX |
Product Type | CHO Expression Medium |
Form | Liquid |
Without Additives | No Phenol Red |
Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
Culture Type | Suspension Cell Culture |
Product Line | ExpiCHO™ |
Serum Level | Serum-free |
Gibco™ DMEM, no glucose, no glutamine, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
A soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. It is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation.


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Shipping Condition | Dry Ice |
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Purity or Quality Grade | Cell Culture Grade |
Form | Frozen |
Without Additives | No Phenol Red |
Endotoxin Level | Low |
Concentration | 12 to 18 mg/mL |
Sterility | Sterile |
Cell Type | Variety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC) |
Format | Tube(s) |
Product Type | Basement Membrane Matrix |
Classification | Reduced Growth Factor |
Culture Type | Adherent Cell Culture |
Shelf Life | 36 months |
Species | Mouse |
Product Line | Gibco™ |
Serum Level | Serum-free |