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Protein Analysis Reagents

A wide variety of products for use in the study of proteins. Includes protein samples, extraction and purification products, protein modifiers, assays, dialysis supplies, gene reporter systems, cDNA, competent cells, immunohistochemistry supplies, etc.
Protein Purification (429)
Crosslinking, Labeling and Protein Modification (64)
Dialysis and Desalting (63)
Protein Resins (28)
Antibody Production and Purification (18)
Immunohistochemistry Kits and Reagents (5)
Protein Mass Spectrometry Sample Prep (5)
Protein Assays (5)
Antibody Affinity Purification Buffers (3)
Streptavidin-Biotin Binding Products (3)
Protein Detection Systems (2)

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1 – 15 of 352 results
1

Cytiva Protein A and Protein G Mag Sepharose™

Simplify enrichment of target proteins by immunoprecipitation or pull-down applications

EDGE
Particle Size 37 to 100 μm
Content And Storage 4°C to 8°C
Matrix Paramagnetic spherical, highly cross linked agarose particles
2
Promotions Available

Cytiva HisTrap™ FF Columns: 16 x 25mm
GSA_VA

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
3

Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns
GSA_VA

Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.

EDGE
Content And Storage 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol
Description HiTrap SP FF
4

Cytiva HiTrap™ NHS-Activated HP Columns
GSA_VA

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

EDGE
Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
5

Cytiva HiTrap™ Protein G HP Prepacked Columns
GSA_VA

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

EDGE
Content And Storage 4°C to 30°C
Description HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
Storage Buffer 20% Ethanol
Product Type Pre-packed Columns
For Use With (Application) Designed for use with a syringe, peristaltic pump, or chromatography system
6

Cytiva HiTrap™ Heparin HP Columns
GSA_VA

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
7
Promotions Available

Cytiva HiTrap™ Desalting Prepacked Columns
GSA_VA

For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.

EDGE
Disposable Reusable
Particle Size >20 μm (dry), 15 to 88 μm (wet)
Content And Storage 4°C to 30°C in 20% Ethanol
Diameter (English) Inner Column 0.62 in.
Description HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
Volume (Metric) Bed 5 mL
For Use With (Application) For rapid convenient desalting and buffer exchange
Diameter (Metric) Inner Column 16 mm
8

Cytiva HisTrap™ HP Prepacked Columns
GSA_VA

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
9

Cytiva HisTrap™ excel Prepacked Columns
GSA_VA

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
10

Cytiva Ni Sepharose™ 6 Fast Flow
GSA_VA

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

EDGE
Particle Size 90μm
Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Flow Rate 600 cm/hr.
Height (Metric) 250mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type Ni Sepharose 6 FF chromatography media
dimMatrix Ni-Sepharose
Support Type 6% Cross-linked Agarose
Max. Pressure 1 bar
Height (English) 9.84 in.
11

Cytiva Ni Sepharose™ excel
GSA_VA

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

EDGE
Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
12

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
13

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

EDGE
14

Cytiva Disposable PD-10 Columns
GSA_VA

Use for gravity flow purification. Disposable PD-10 Columns are economical, plastic columns that can be packed with a wide range of bulk media.

EDGE
15
Promotions Available

Cytiva Disposable PD-10 Desalting Columns
GSA_VA

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

EDGE