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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 46 results
1

Cytiva HiLoad™ Superdex™ 200 PG XK Column

Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.

Content And Storage 4 to 30°C, 20% Ethanol
2

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
GSA_VA

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Particle Size 25 to 75 μm
Height (Metric) 60cm
pH Range 3 to 11
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity Each
Material Polypropylene
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
3

Cytiva Superdex™ 200 Prep Grade
GSA_VA

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

EDGE
4

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
5

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
GSA_VA

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
6

Cytiva HiTrap™ NHS-Activated HP Columns
GSA_VA

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

EDGE
Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
7

Cytiva HiTrap™ Heparin HP Columns
GSA_VA

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
8

Cytiva HiPrep™ Sephacryl™ S-100 HR Columns

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Peptides and small proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
9

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Particle Size 34 μm
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 4 to 12
Product Type Blue HP Columns
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins
Material Polypropylene
For Use With (Application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
10

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns
GSA_VA

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
11

Cytiva HiPrep™ Sephacryl™ S-300 HR Columns
GSA_VA

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4° to 30°C
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Max. Pressure 22psi
Height (English) 23.62 in.
12

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
13

Cytiva Sephadex™ LH-20
GSA_VA

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
14

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Content And Storage 4° to 30°C
Bed Volume 15 to 20mL/g in Distilled Water
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-100 Media
Sample Type Large biomolecules
Quantity 100 g
For Use With (Application) Useful for desalting and Buffer exchange of very large biomolecules
Support Type Cross-linked Dextran
Max. Pressure 0.096 bar
15

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose