Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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1 – 11 of 11 results

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin
Cut Site	C.TNAG
Content And Storage	Keep container tightly closed
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI

OPTIZYME™ RsaI, Fisher BioReagents™

Pricing & Availability

Components	25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
Cut Site	G.AATTC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	EcoRI
Storage Buffer	10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI

OPTIZYME™ AloI, Fisher BioReagents™

Pricing & Availability

OPTIZYME™ NotI, Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin
Cut Site	GC.GGCCGC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	NotI
Storage Buffer	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Concentration	10 U/μL
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

OPTIZYME™ HindIII, Fisher BioReagents™

Pricing & Availability
Specifications

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site	A.AGCTT
Content And Storage	Keep container tightly closed
Format	Liquid
Product Type	HindIII
Storage Buffer	10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site	G.GATCC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	BamHI
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI

OPTIZYME™ Csp6I (CviQI), Fisher BioReagents™

Pricing & Availability

OPTIZYME™ XbaI, Fisher BioReagents™

Pricing & Availability
Specifications

Components	10X OPTIZYME™ Buffer 4
Cut Site	T.CTAGA
Form	Liquid
Product Type	XbaI
Storage Buffer	10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme

Restriction Enzymes

Restriction Enzymes

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