Protein Extraction and Purification

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits Available on GSA/VA Contract for Federal Government customers only.

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific™ Pierce™ Protein A/G Agarose and Kits Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with versatile, recombinant Protein A/G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

Thermo Scientific™ Pierce™ NHS-Activated Agarose

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

BD Recombinant Human β2 Microglobulin

Recombinant protein expressed in insect cells and purified by a combination of ion-exchange and gel-filtration methods

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Pierce™ Kinase Enrichment Kits and Probes

Desthiobiotin-ATP and -ADP probes to label, affinity purify and measure the active site of ATPases (kinases), including chaperones and metabolic enzymes.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ Pierce™ High-Capacity Protein L Agarose

Purify antibodies containing kappa light chains or perform immunoprecipitation with this high-capacity Protein L resin on beaded agarose.

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ GlycoLink™ Immobilization Kits and Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates Available on GSA/VA Contract for Federal Government customers only.

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ Pierce™ HA Tag IP/Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Pierce™ Magnetic ChIP Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

EMD Millipore™ BioPak™ Disposable Ultrafilter Available on GSA/VA Contract for Federal Government customers only.

Nuclease-free, pyrogen-free water from any EMD Millipore Ultrapure™ water system

Invitrogen™ Total Exosome Isolation Reagent (from cell culture media) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Easily isolate exosomes and avoid time-consuming ultra-centrifugation

Thermo Scientific™ Pierce™ RIPA Buffer Available on GSA/VA Contract for Federal Government customers only.

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ HisPur™ Ni-NTA Resin Available on GSA/VA Contract for Federal Government customers only.

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits Available on GSA/VA Contract for Federal Government customers only.

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ CarboxyLink™ Coupling Resins and Immobilization Kits

Covalently immobilize peptide antigens and other carboxylate ligands for affinity purification using DADPA-activated agarose beads and EDC crosslinker.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Invitrogen™ Molecular Probes™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution

Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Perform immunoprecipitation (IP) assays of HA-tagged proteins or co-IP reactions with HA-tagged bait proteins with these magnetic beads, positive control and reagents.

Thermo Scientific™ Pierce™ Streptavidin Resins Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution Available on GSA/VA Contract for Federal Government customers only.

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Protein L Agarose and Kits

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns Available on GSA/VA Contract for Federal Government customers only.

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

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