Modification Reagents for Amino Acids
Thermo Scientific™ Pierce™ N-Ethylmalemde (NEM)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific™ Pierce™ Traut's Reagent
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ Pierce™ SATA
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ Pierce™ SAT(PEG)4
Long (18.2A), pegylated form of SATA, an amine-reactive (NHS ester) sulfhydryl-addition reagent; contains 4-unit polyethylene glycol spacer arm.
Thermo Scientific™ Pierce™ MMTS
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Thermo Scientific™ Pierce™ Crystalline ACS Grade Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ Hydroxylamine·HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Thermo Scientific™ GlycoLink™ Coupling Catalyst
Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.
Thermo Scientific™ Pierce™ SATP
Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ AEDP Crosslinker
Crosslink amines to carboxyls with this spacer containing an internal disulfide bond; use EDC to modify functional groups on proteins and surfaces.
Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Thermo Scientific™ Pierce™ TFCS
Modify proteins by adding a 7-atom spacer arm with this amine-reactive (NHS ester) compound; the terminal trifluoroacetyl group can be removed to expose a new primary amine.
Thermo Scientific™ Pierce™ EMCA
Permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives) using this sulfhydryl-reactive (maleimide) compound.
Thermo Scientific™ Pierce™ Citraconic Anhydride
Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).