Protein Assays and Amino Acid Analysis
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
BD BD GolgiStop™ Protein Transport Inhibitor (containing Monensin)
Block the intracellular protein transport processes when added to in vitro- or in vivo-stimulated lymphoid cells
BD GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A)
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
Promega™ Isoquant™ Isoaspartate Detection Kit
Quantitatively detects isoaspartate resulting from aspartic acid rearrangement as well as deamidation of asparagine
Invitrogen™ NA-Fluor™ Influenza Neuraminidase Assay Kit
An assay for monitoring neuraminidase enzyme activity from mixed viral populations, non-viral or bacterial sources. Thermo Scientific NA-Fluor Influenza Neuraminidase Assay Kit provides validated reagents and standardized protocol for conducting neuramini
Promega™ BCIP/NBT Color Development Substrate
For the colorimetric detection of alkaline phosphatase activity
Promega™ Amino Acid Mixture Minus Leucine
1mM Solution of the 20 essential amino acids minus Leucine.
Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection
Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.
R&D Systems™ NEDD8 Conjugation Initiation Kit
Designed for the conjugation of the ubiquitin-like modifier NEDD8 to protein substrates in vitro
Applied Biosystems™ cAMP-Screen Direct™ Cyclic AMP Immunoassay System, 96-well
Ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates with a simplified protocol. Thermo Scientific cAMP-Screen Direct 96-well Cyclic AMP Immunoassay System is a competitive chemiluminescent immunoassay formatted with maximum flexibilit
R&D Systems™ Human CHIP Ubiquitin Ligase Kit, Glow-Fold Substrate
Designed for in vitro ubiquitination of user-supplied substrates by CHIP or the CHIP/HSP70/HSP40 ternary complex
Applied Biosystems™ cAMP-Screen™ Cyclic AMP Immunoassay System, 96-well
Ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates. Thermo Scientific cAMP-Screen™ 96-well Cyclic AMP immunoassay System is a competitive chemiluminescent immunoassay formatted with maximum flexibility to permit either manual r
Invitrogen™ I-Block™ Protein-Based Blocking Reagent
Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.
GE Healthcare Amersham™ ECL Advance™ Blocking Reagent
Use for Controlling background noise. GE Healthcare Life Sciences™ Amersham™ ECL Advance™ is Blocking Reagent controls background noise while retaining a high level of signal intensity.
Promega MAO-Glo™ Monoamine Oxidase Assay System
The MAO-Glo™ Assay provides a homogeneous luminescent method for measuring monoamine oxidase activity from recombinant and native sources and for testing the effects of test compounds on MAO activity.
GE Healthcare 2-D Quant Kit
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. GE Healthcare Life Sciences™ 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
R&D Systems™ 20S Proteasome Assay Kit, SDS Activation Format
Designed for the quantitative analysis of 20S proteasome activity in cuvets or 96-well microplates
Invitrogen™ Development Folders
For use during imaging of blotting membranes following chemiluminescent signal development (western blotting, nucleic acid blotting).
R&D Systems™ Human MDM2/S5a Ubiquitination Kit
Designed for in vitro MDM2-mediated ubiquitination of user-supplied substrates such as p53, beta-Arrestin and DYRK
R&D Systems™ Human E6AP/S5a Ubiquitination Kit
Designed for in vitro E6AP-mediated ubiquitination of user-supplied, reported substrates such as β-Arrestin, and DYRK2
Invitrogen™ 10X Assay Buffer
Used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates.
Invitrogen™ Sapphire-II™ Luminescence Enhancer for Solution Assays
Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.
R&D Systems™ Ubiquitin Conjugation Initiation Kit
Designed to charge ubiquitin by E1, the first reaction in the conjugation cascade