Proteomics

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ Hydrochloric Acid Solution Available on GSA/VA Contract for Federal Government customers only.

Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.

Thermo Scientific™ SuperBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Thermo Scientific™ Zeba Spin 7K MWCO Desalting Columns Available on GSA/VA Contract for Federal Government customers only.

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions Available on GSA/VA Contract for Federal Government customers only.

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce™ Disposable Plastic Columns Available on GSA/VA Contract for Federal Government customers only.

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce™ PNPP Substrates

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ Pierce™ Sulfo-LC-SPDP Crosslinker

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit Available on GSA/VA Contract for Federal Government customers only.

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

Thermo Scientific™ Slide-A-Lyzer™ 10K MWCO MINI Dialysis Devices Available on GSA/VA Contract for Federal Government customers only.

Perform sample buffer exchange with these 10,000-MW cut-off (MWCO) disposable polypropylene cups with integrated, low-binding membranes.

Thermo Scientific™ Pierce™ Citraconic Anhydride

Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).

Thermo Scientific™ Pierce™ TMB Substrates Available on GSA/VA Contract for Federal Government customers only.

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay Available on GSA/VA Contract for Federal Government customers only.

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Zeba™ Spin Desalting Plates

Perform high-throughput sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening and assay development.

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Thermo Scientific™ Pierce™ 660nm Protein Assay Available on GSA/VA Contract for Federal Government customers only.

Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Pierce™ High Binding Streptavidin Coated Plates Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available streptavidin-coated plates.

Thermo Scientific™ Pierce™ S6 In-Cell ELISA Kit

Measure total and phosphorylated ribosomal S6 protein (phospho-S6) using S6 antibodies detected by fluorescence or TMB substrate in these whole-cell microplate assays.

Thermo Scientific™ Slide-A-Lyzer™ 20K MWCO G2 Dialysis Cassettes Available on GSA/VA Contract for Federal Government customers only.

Remove buffer salts and contaminants while maximizing sample recovery with these second-generation self-floating protein dialysis cassettes (20K MWCO).

Thermo Scientific™ Pierce™ Micro BCA™ Protein Assay Available on GSA/VA Contract for Federal Government customers only.

Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

Thermo Scientific™ Pierce™ Biotin Quantitation Kit Available on GSA/VA Contract for Federal Government customers only.

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Slide-A-Lyzer™ 3.5K MWCO G2 Dialysis Cassettes Available on GSA/VA Contract for Federal Government customers only.

Remove buffer salts and contaminants while maximizing sample recovery with these second-generation self-floating protein dialysis cassettes (3.5K MWCO).

Thermo Scientific™ Pierce™ Inclusion Body Solubilization Reagent

Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.

Thermo Scientific™ Pierce™ BCA™ Protein Assay Available on GSA/VA Contract for Federal Government customers only.

Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Thermo Scientific™ Slide-A-Lyzer™ 10K MWCO Dialysis Cassettes Available on GSA/VA Contract for Federal Government customers only.

Remove buffer salts and contaminants and maximize sample recovery using this semi-permeable membrane with a 10000-dalton protein MW cut-off (MWCO).

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