DNA Cloning and Mapping and Mutagenesis

Thermo Scientific™ CloneJET™ PCR Cloning Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Thermo Scientific™ Rapid DNA Ligation Kit Available on GSA/VA Contract for Federal Government customers only.

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Invitrogen™ GeneRacer™ SuperScript™ TA Cloning™ TOPO™ GeneRacer Kit with SuperScript III RT and TOPO TA Cloning™ Kit for Sequencing Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process

Promega ImProm-II™ Reverse Transcription

Formulated for stand-alone applications or for easy transition to gene-specific target amplification.

Invitrogen™ GeneArt™ Seamless Cloning and Assembly Enzyme Mix Non-distribution product as customer accommodation.

Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction

Promega JM109 Competent Cells

JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening.

Invitrogen™ GeneArt™ Site-Directed Mutagenesis PLUS System Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Brings our mutagenesis technology to the next level with the ability to perform single- or multi-site mutations in larger plasmids with greater efficiency and in less time than the competition

GE Healthcare Ready-to-Go™ You-Prime First-Strand Beads

Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. GE Healthcare Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.

Invitrogen™ GeneArt™ Site-Directed Mutagenesis System. Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Make substitutions, deletions, or insertions of up to 12 nucleotides in plasmids up to 14kb in vitro

Invitrogen™ GeneArt™ Seamless Cloning and Assembly Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction

Applied Biosystems™ MicroAmp™ 96-Well Base Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Convenient for pre- and post-PCR sample processing

Invitrogen™ 3' RACE System for Rapid Amplification of cDNA Ends Non-distribution product as customer accommodation.

Converts up to 1μg of total RNA preparation or 100ng of control RNA into cDNA in one reaction

Thermo Scientific™ Lambda DNA Available on GSA/VA Contract for Federal Government customers only.

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

Invitrogen™ FirstChoice™ RLM-RACE Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Thermo Scientific™ pBR322 DNA Available on GSA/VA Contract for Federal Government customers only.

Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.

Promega HB101 Competent Cells

HB101 cells are recA- to minimize recombination events and contain a restriction-minus background (hsdS20) to allow better representation when cloning methylated DNA. Suitable for vectors that do not require alpha-complementation for blue/white screening.

Invitrogen™ TA Cloning™ Kits with pCR™ 2.1 Vector, without Competent Cells Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Invitrogen™ GeneArt™ Seamless PLUS Cloning and Assembly Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

The complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction

Promega HaloTag™ Interchangeable Labeling Technology

The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.

Invitrogen™ 5' RACE System for Rapid Amplification of cDNA Ends, Version 2.0 Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Suitable for rapid amplification of cDNA ends (RACE) between defined point in mRNA and the 5' end

Thermo Scientific™ E.coli Genomic DNA

Purified from E. coli type B cells, ATCC 11303 strain.

Thermo Scientific™ pUC18, pUC19 DNA Available on GSA/VA Contract for Federal Government customers only.

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

Thermo Scientific™ Lambda DNA (dam-, dcm-) Available on GSA/VA Contract for Federal Government customers only.

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

Thermo Scientific™ pUC18, pUC19 DNA Available on GSA/VA Contract for Federal Government customers only.

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

Thermo Scientific™ pUC57 DNA Available on GSA/VA Contract for Federal Government customers only.

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

Invitrogen™ TA Cloning™ Kit, Dual Promoterwith pCR™ 2.1 Vector without Competent Cells Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Applied Biosystems™ MicroAmp™ 96-Well Reaction Tube, Tray and Retainer Set Non-distribution product as customer accommodation.

Designed for precise PCR on Applied Biosystems 0.2mL thermal cyclers

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