Cell Lysis and Protein Extraction

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ RIPA Buffer Available on GSA/VA Contract for Federal Government customers only.

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ NP-40 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis Available on GSA/VA Contract for Federal Government customers only.

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Thermo Scientific™ Pierce™ Protein Refolding Kit

Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit Available on GSA/VA Contract for Federal Government customers only.

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent Available on GSA/VA Contract for Federal Government customers only.

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Thermo Scientific™ Pierce™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

Thermo Scientific™ Pierce™ IP Lysis Buffer Available on GSA/VA Contract for Federal Government customers only.

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

Thermo Scientific™ Pierce™ CHAPS Available on GSA/VA Contract for Federal Government customers only.

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent Available on GSA/VA Contract for Federal Government customers only.

Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent.

Thermo Scientific™ Pierce™ n-Dodecyl-β-Maltoside Available on GSA/VA Contract for Federal Government customers only.

Water-soluble, non-denaturing and nonionic detergent especially useful for solubilizing hydrophobic membrane proteins to preserve their activity.

Thermo Scientific™ Pierce™ Inclusion Body Solubilization Reagent

Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.

GE Healthcare HiScreen™ MabSelect™, Xtra™, SuRe™, SuRe LX™ Prepacked Columns

Designed for method optimization and parameter screening for capture of monoclonal antibodies.   GE Healthcare HiScreen™ MabSelect™, Xtra™, SuRe™, SuRe LX™ Prepacked Columns come ready-to-use  for convenience.

Thermo Scientific™ Pierce™ B-PER™ Bacterial Protein Extraction Reagents Available on GSA/VA Contract for Federal Government customers only.

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

GE Healthcare XK Chromatography Columns (Empty) Available on GSA/VA Contract for Federal Government customers only.

Ensure trouble-free operation for low to medium pressure. GE Healthcare XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

Thermo Scientific™ Pierce™ DNase I

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

GE Healthcare PD MultiTrap™ Plates

Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with high reproducibility from well to well and from plate to plate

MilliporeSigma™ Bugbuster™ Master Mix Available on GSA/VA Contract for Federal Government customers only.

Conveniently combined extraction reagents in a single solution. MilliporeSigma™; Bugbuster™ Master Mix allows for maximum recovery of active soluble protein from both Gram-negative/Gram-positive bacteria.

Promega Nano-Glo™ Luciferase Assay System

The Nano-Glo™ Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.

Thermo Scientific™ Pierce™ Micrococcal Nuclease Available on GSA/VA Contract for Federal Government customers only.

Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.

GE Healthcare PreScission™ Protease Available on GSA/VA Contract for Federal Government customers only.

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. GE Healthcare Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

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