Protein Affinity Purification

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

GE Healthcare Glutathione Sepharose™ 4B Media Available on GSA/VA Contract for Federal Government customers only.

Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. GE Healthcare Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.

GE Healthcare Sephaorose™ Fast Flow Anion Exchange Media: DEAE

Used for preparative protein separations in both research and industrial applications.  GE Healthcare Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.

GE Healthcare MabSelect Sure™ Chromatography Media Available on GSA/VA Contract for Federal Government customers only.

MabSelect™ SuRe™ is a protein A affinity medium for capturing MAbs at process scale with an alkali-tolerant rProtein A ligand that allows the for cost-effective sanitization protocols

GE Healthcare Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media Available on GSA/VA Contract for Federal Government customers only.

USe for  separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins.  GE Healthcare Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.

GE Healthcare HiTrap™ MabSelect™ Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. GE Healthcare Life Sciences™ HiTrap™ MabSelect™ Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

GE Healthcare Benzamidine Sepharose™ 4 Fast Flow, High Sub Media Available on GSA/VA Contract for Federal Government customers only.

For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases and thrombin

GE Healthcare Sephaorose™ Fast Flow Anion Exchange Media: Q Available on GSA/VA Contract for Federal Government customers only.

Use for preparative protein separations in both research and industrial applications. GE Healthcare Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.

GE Healthcare Phenyl Sepharose™ 6 Fast Flow Chromatography Media Available on GSA/VA Contract for Federal Government customers only.

Used for capture and intermediate purification requiring low to medium hydrophobicity

MilliporeSigma™ Calbiochem Hydroxylapatite, Fast Flow

A form of calcium phosphate used in preparative procedures for purification of proteins and nucleic acids in which high flow rate and binding capacity are desired. BSA binding capacity: >12mg/g; DNA binding capacity: >700μg/g

GE Healthcare CNBr-Activated Sepharose™ 4 Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Used for coupling of large amino-containing ligands

GE Healthcare Capto™ Adhere Media

A multimodal anion exchanger to follow the Protein A capture step, making for a streamlined process

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

GE Healthcare nProtein A Sepharose™ 4 Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Used for purification of monoclonal and polyclonal antibodies.   GE Healthcare Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

GE Healthcare Sepharose™ Fast Flow Cation Exchange Media Available on GSA/VA Contract for Federal Government customers only.

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

GE Healthcare rProtein A Sepharose™ Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

GE Healthcare Life Sciences rProtein A GraviTrap™

Includes ten ready-to-use columns prepacked with 1ml of rProtein A Sepharose Fast Flow

GE Healthcare NHS-Activated Sepharose™ 4 Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Use for coupling amino-containing smaller proteins and peptides.  GE Healthcare Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

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