Protein Mass Spectrometry and HPLC

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

For multiplex quantitative protein expression analysis by mass spectrometry

Thermo Scientific™ RPMI 1640 Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by quantitative mass spectrometry using amine-labeling compounds of equal total mass (isobaric) but different reporter ions.

Thermo Scientific™ Pierce™ HeLa Protein Digest Standard Available on GSA/VA Contract for Federal Government customers only.

Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Thermo Scientific™ Pierce™ ESI Negative Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Thermo Scientific™ DMEM Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ Hydrochloric Acid Solution

Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.

Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce™ MS Grade Trypsin Protease Available on GSA/VA Contract for Federal Government customers only.

Ensure maximum activity and stability in proteomic applications with this chemically modified, highly purified porcine trypsin.

Thermo Scientific™ Pierce™ C-18 Spin Columns

Desalt, clean up and prep 25 to 150 microliter peptide samples for mass spectrometry with these ready-to-use microcentrifuge columns of C18 reverse-phase resin.

Thermo Scientific™ Pierce™ Amino Acid Standard H

Calibrate HPLC analysis of protein hydrolysates using this calibration standard mixture of 18 amino acids, each at 2.5 micromoles/mL in 0.1N HCl.

Thermo Scientific™ Pierce™ Cysteine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Measure total peptide concentration by a colorimetic microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay.

Thermo Scientific™ SILAC Protein Quantitation Kit (LysC) - DMEM

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ High-Select™ Phosphopeptide Enrichment Kit

Enriches phosphopeptides from complex samples.

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit.

Thermo Scientific™ Pierce™ LTQ ESI Positive Ion Calibration Solution

Calibrate LTQ, LTQ Orbitrap, LXQ and LCQ FLEET mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ Pierce™ C-18 Tips Available on GSA/VA Contract for Federal Government customers only.

Capture, concentrate and desalt 10 to 100 microliter samples of peptides and proteins for MALDI mass spectrometry with these ready-to-use pipette-tip columns of C18 resin.

Thermo Scientific™ Pierce™ Peptide Retention Time Calibration Mixture

15 synthetic heavy peptides mixed in equimolar ratio to test and calibrate reverse-phase liquid chromatography (LC) and mass spectrometry (LC-MS) equipment.

Thermo Scientific™ Pierce™ Abundant Protein Depletion Spin Columns Available on GSA/VA Contract for Federal Government customers only.

Decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or 1D and 2D gel electrophoresis.

Thermo Scientific™ Pierce™ Quantitative Fluorometric Peptide Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Measure total peptide concentration by a mix-and-read fluorescent microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay.

Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution

Calibrate Triple Stage Quadrupole (TSQ) mass spectrometers using a mixture of high purity, ionizable components specifically designed for positive mode calibration.

Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution, Extended Mass Range

Obtain both positive and negative ionization calibration of Thermo Scientific™ Triple Stage Quadrupole instruments using the Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution, Extended Mass Range, which is a mixture of 14 highly pure, ionizable components (mass range: 69 m/z to 2800m/z).

Thermo Scientific™ Pierce™ Protein Precipitation Plates Available on GSA/VA Contract for Federal Government customers only.

Quickly precipitate and remove proteins from serum or other biological fluids to purify small molecules for mass spectrometry using these 96-well filter plates.

Thermo Scientific™ Pierce™ Albumin Depletion Kit Available on GSA/VA Contract for Federal Government customers only.

The Thermo Scientific™ Pierce Albumin Depletion Kit uses an agarose resin of the affinity ligand Cibacron Blue dye for high-capacity, selective extraction of human serum albumin from 10 to 50μL serum samples.

Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade

Calibrate and maintain quality control for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples using the carefully validated Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade.

Promega MS Compatible Human Protein Extract, Digest

Mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spec analysis

Promega 6 x 5 LC-MS/MS Peptide Reference Mix

The 6 x 5 LC-MS/MS Peptide Reference Mix is a unique reagent designed to monitor liquid chromatography (LC) and mass spectrometry (MS) instrument performance and assist in method development and optimization.

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