Buffers and Diluents

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Non-distribution product as customer accommodation.

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ SuperBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

BD Brilliant Stain Buffer

Designed to complement multicolor flow cytometry experiments. Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells.

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

R&D Systems™ Reagent Diluent Concentrate 2, 10X Available on GSA/VA Contract for Federal Government customers only.

10X Concentrated 10% BSA Solution for DuoSets

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Thermo Scientific™ 6X DNA Loading Dye Available on GSA/VA Contract for Federal Government customers only.

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ Novex™ NuPAGE™ Antioxidant Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

GE Healthcare Pharmalyte™ Carrier Ampholytes Available on GSA/VA Contract for Federal Government customers only.

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ eBioscience™ Permeabilization Buffer, 10x Non-distribution product as customer accommodation.

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Solutions Non-distribution product as customer accommodation.

For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.

R&D Systems™ Stop Solution 2N Sulfuric Acid Available on GSA/VA Contract for Federal Government customers only.

For stopping the action of HRP on the substrate, tetramethylbenzidine

Thermo Scientific™ Pierce™ SEA BLOCK™ Blocking Buffer Available on GSA/VA Contract for Federal Government customers only.

Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Contains the NativePAGE Running Buffer (20X)

R&D Systems™ Quantikine Wash Buffer 1 Available on GSA/VA Contract for Federal Government customers only.

Concentrate that can be diluted to a working concentration and used for the complete removal of any unbound sample or conjugate in the wells of an ELISA

Thermo Scientific™ Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ Novex™ Bolt™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

BD OptEIA™ Assay Diluent Available on GSA/VA Contract for Federal Government customers only.

A buffered protein solution containing fetal bovine serum that is intended for use in BD OptEIA ELISA Sets

Invitrogen™ Novex™ NativePAGE™ Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Used to make the NativePAGE Cathode and Anode Running Buffers

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ eBioscience™ Intracellular (IC) Fixation Buffer Non-distribution product as customer accommodation.

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

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