Buffers and Diluents

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Non-distribution product as customer accommodation.

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ SuperBlock™ (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

BD Brilliant Stain Buffer

Designed to complement multicolor flow cytometry experiments. Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells.

Thermo Scientific™ SuperBlock™ T20 (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Thermo Scientific™ SuperBlock™ (TBS) Blocking Buffer Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Thermo Scientific™ SEA BLOCK Blocking Buffer

Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.

Thermo Scientific™ SuperBlock™ T20 (TBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Cytiva (Formerly GE Healthcare Life Sciences) Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

R&D Systems™ Reagent Diluent Concentrate 2, 10X Available on GSA/VA Contract for Federal Government customers only.

10X Concentrated 10% BSA Solution for DuoSets

Thermo Scientific™ StartingBlock™ T20 (PBS) Blocking Buffer Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Invitrogen™ eBioscience™ Permeabilization Buffer (10X) Non-distribution product as customer accommodation.

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

R&D Systems™ Stop Solution 2N Sulfuric Acid Available on GSA/VA Contract for Federal Government customers only.

For stopping the action of HRP on the substrate, tetramethylbenzidine

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Invitrogen™ NuPAGE™ Antioxidant Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Thermo Scientific™ DNA Gel Loading Dye (6X) Green Product Available on GSA/VA Contract for Federal Government customers only.

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ StartingBlock™ (PBS) Blocking Buffer

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Thermo Scientific™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Non-distribution product as customer accommodation.

For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.

Invitrogen™ Bolt™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

R&D Systems™ Quantikine Wash Buffer 1 Available on GSA/VA Contract for Federal Government customers only.

Quantikine ELISA Wash Buffer 1 from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Invitrogen™ eBioscience™ IC Fixation Buffer Non-distribution product as customer accommodation.

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ Digitonin (5%) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

A detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

BD OptEIA™ Assay Diluent

A buffered protein solution containing fetal bovine serum that is intended for use in BD OptEIA ELISA Sets

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