Buffers and Diluents

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Non-distribution product as customer accommodation.

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

BD Brilliant Stain Buffer

Designed to complement multicolor flow cytometry experiments. Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells.

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ SuperBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

R&D Systems™ Reagent Diluent Concentrate 2, 10X Available on GSA/VA Contract for Federal Government customers only.

10X Concentrated 10% BSA Solution for DuoSets

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Thermo Scientific™ 6X DNA Loading Dye Available on GSA/VA Contract for Federal Government customers only.

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Invitrogen™ Novex™ NuPAGE™ Antioxidant Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

R&D Systems™ Stop Solution 2N Sulfuric Acid Available on GSA/VA Contract for Federal Government customers only.

For stopping the action of HRP on the substrate, tetramethylbenzidine

GE Healthcare Pharmalyte™ Carrier Ampholytes Available on GSA/VA Contract for Federal Government customers only.

Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ eBioscience™ Permeabilization Buffer, 10x Non-distribution product as customer accommodation.

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Thermo Scientific™ Pierce™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent

Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Solutions Non-distribution product as customer accommodation.

For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Made with high-purity reagents and are strictly quality controlled

R&D Systems™ Quantikine Wash Buffer 1 Available on GSA/VA Contract for Federal Government customers only.

Concentrate that can be diluted to a working concentration and used for the complete removal of any unbound sample or conjugate in the wells of an ELISA

Invitrogen™ Novex™ NativePAGE™ Running Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Used to make the NativePAGE Cathode and Anode Running Buffers

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Contains the NativePAGE Running Buffer (20X)

Thermo Scientific™ Pierce™ SEA BLOCK™ Blocking Buffer Available on GSA/VA Contract for Federal Government customers only.

Block mammalian tissue samples for immunohistochemistry (IHC) and samples for other methods with steelhead salmon serum in PBS.

Invitrogen™ eBioscience™ Intracellular (IC) Fixation Buffer Non-distribution product as customer accommodation.

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ Novex™ Bolt™ Transfer Buffer (20X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Optimized for the transfer of proteins from Bolt Bis-Tris Plus gels to membranes for western blotting

Invitrogen™ Novex™ IEF Cathode Buffer pH 3-10 (10X) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

An optimized pre-mixed IEF cathod buffer

BD Perm / Wash™ Buffer I (10x) Available on GSA/VA Contract for Federal Government customers only.

Intracellular staining of post-translationally modified signaling proteins

Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent Non-distribution product as customer accommodation.

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

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