Western Blotting Reagents, Membranes, Supplies

Thermo Scientific™ Pierce™ Recombinant Protein A/G and Conjugates

Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.

Alfa Aesar™ Caspase-3 Substrate V, fluorogenic

For cellular and molecular biology applications

Alfa Aesar™ [Leu8] Renin Substrate Tetradecapeptide, Rat

For cellular and molecular biology applications

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ CL-XPosure™ Film (X-Ray Film)

Capture results of ECL-based Western blots and other chemiluminescent protein or nucleic acid assays using this economically priced, clear-blue X-ray film.

Thermo Scientific™ Pierce™ NeutrAvidin™ High Sensitivity Protein HRP Conjugates

Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.

Thermo Scientific™ Pierce™ SuperSignal West Dura Fast Western Blot Kits

For femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Blocker™ BLOTTO Blocking Buffer

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ Pierce™ Western Blotting Filter Papers

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Thermo Scientific™ myImageAnalysis™ Software

myImageAnalysis Software is a full-featured, multifunction computer program to analyze and edit digital images of electrophoresis gels and blots that were acquired using gel documentation imagers or scanners.

Thermo Scientific™ Pierce™ PVDF Membranes

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

Thermo Scientific™ Pierce™ Peroxidase Conjugate Stabilizer

An antifreeze solution to store aliquots and stocks of HRP-conjugated antibodies so they can be pipetted for use without thawing; better than glycerol.

Alfa Aesar™ Elastase Substrate IV, Colorimetric

For cellular and molecular biology applications

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Thermo Scientific™ Lab Vision™ Ready-To-Use AEC Substrate System

Utilize 3-Amino-9-Ethylcarbazole (AEC), a widely used chromogen for immunohistochemical staining with the Thermo Scientific™ Lab Vision™ AEC Substrate System. 

Thermo Scientific™ Pierce™ Nylon Membranes

Sheets of unmodified or positively charged Biodyne A and Biodyne B Nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ myECL™ Imager Accessories and Replacement Parts

Document and analyze images of blots and gels using a sensitive and easy-to-use system with an intuitive touchscreen interface and advanced integrated software.

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Thermo Scientific™ Pierce™ SuperSignal West Pico Fast Western Blot Kit

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Alfa Aesar™ HIV Substrate

For cellular and molecular biology applications

Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer/DAB Plus Chromogen

Take advantage of a senstive detection system based on biotin-free, polymer technology.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ Pierce™ Background Eliminator for Film

Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.

Alfa Aesar™ Chymotrypsin Substrate III, fluorogenic

For cellular and molecular biology applications

Alfa Aesar™ [Cys8] Renin Substrate Tetradecapeptide, Rat

For cellular and molecular biology applications

Alfa Aesar™ Caspase-3 Substrate III, fluorogenic

For cellular and molecular biology applications

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

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