Enzymes and Inhibitors

Thermo Scientific™ FastDigest™ SacI Available on GSA/VA Contract for Federal Government customers only.

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI)

Thermo Scientific™ AluI Available on GSA/VA Contract for Federal Government customers only.

Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).

Thermo Scientific™ FastDigest™ SacI Promotion

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI)

Thermo Scientific™ RiboLock RNase Inhibitor Available on GSA/VA Contract for Federal Government customers only.

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Thermo Scientific™ BcuI (SpeI) Available on GSA/VA Contract for Federal Government customers only.

Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI).

Thermo Scientific™ FastDigest™ PstI Available on GSA/VA Contract for Federal Government customers only.

The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).

Thermo Scientific™ Agarase Available on GSA/VA Contract for Federal Government customers only.

Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.

Thermo Scientific™ FastDigest PdmI Available on GSA/VA Contract for Federal Government customers only.

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ Alw21I (BsiHKAI) Available on GSA/VA Contract for Federal Government customers only.

Cut at GWGCW^C sites with Alw21I (BsiHKAI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: Bbv12I, BsiHKAI).

Thermo Scientific™ FastDigest™ PstI Promotion

The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).

Thermo Scientific™ FastDigest™ DraIII (AdeI) Promotion

Cut at CACNNN^GTG sites with Thermo Scientific™ FastDigest™ DraIII (AdeI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ Proteinase K (recombinant), PCR grade Available on GSA/VA Contract for Federal Government customers only.

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Thermo Scientific™ AatII Available on GSA/VA Contract for Federal Government customers only.

Cut at GACGT^C sites with AatII restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ZraI).

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Thermo Scientific™ BseMI (BsrDI) Available on GSA/VA Contract for Federal Government customers only.

Cut at GCAATG(2/0)^ sites with BseMI (BsrDI) restriction enzyme, which performs best at 55°C in R buffer (Isoschizomers: Bse3DI, BsrDI).

Thermo Scientific™ NcoI Available on GSA/VA Contract for Federal Government customers only.

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).

Thermo Scientific™ FastDigest™ PdmI Promotion

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ Klenow Fragment, exo- Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Thermo Scientific™ SspDI (KasI) Available on GSA/VA Contract for Federal Government customers only.

Cut at G^GCGCC sites with SspDI (KasI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI).

Thermo Scientific™ DNA Polymerase I Available on GSA/VA Contract for Federal Government customers only.

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Thermo Scientific™ Alw26I (BsmAI) Available on GSA/VA Contract for Federal Government customers only.

Cut at GTCTC(1/5)^ sites with Alw26I (BsmAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsmAI, BstMAI).

Thermo Scientific™ FastDigest TaiI Available on GSA/VA Contract for Federal Government customers only.

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest SwaI (SmiI) Available on GSA/VA Contract for Federal Government customers only.

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ Bsu15I (ClaI) Available on GSA/VA Contract for Federal Government customers only.

Cut at AT^CGAT sites with Bsu15I (ClaI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BanIII, Bsa29I, BseCI, BshVI, BspDI, BspXI, BsuTUI).

Thermo Scientific™ SchI (MlyI) Available on GSA/VA Contract for Federal Government customers only.

Cut at GAGTC(5/5)^ sites with SchI (MlyI) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: MlyI, PleI, PpsI, SchI).

Thermo Scientific™ Buffer EcoRI Available on GSA/VA Contract for Federal Government customers only.

Optimize digestions with EcoRI restriction enzyme using this buffer, premixed with BSA for enhanced stability.

Thermo Scientific™ FastDigest™ Eco32I Available on GSA/VA Contract for Federal Government customers only.

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).

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