Miscellaneous Reagents for Molecular Biology

Thermo Scientific™ Pierce™ Phenol: Chloroform

Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.

Thermo Scientific™ IPTG Available on GSA/VA Contract for Federal Government customers only.

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Thermo Scientific™ X-Gal Available on GSA/VA Contract for Federal Government customers only.

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Thermo Scientific™ Pierce™ Phenol Saturated

Perform typical molecular biology applications with this saturated phenol solution (pH 6.6), accompanied by a buffer to adjust the pH to 7.9.

Thermo Scientific™ 5-Fluoroorotic Acid

Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.

Thermo Scientific™ Aminoallyl-UTP Available on GSA/VA Contract for Federal Government customers only.

Perform enzymatic indirect non-radioactive labeling of RNA during in vitro transcription with the modified nucleotide Aminoallyl-UTP.

Thermo Scientific™ DTT

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

Thermo Scientific™ Pierce™ Sodium Acetate Solution

Consitently purify and store DNA without any worries using this highly purified, sterile 3M sodium acetate solution.

Thermo Scientific™ Pierce™ Yeast DNA Extraction Kit

Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.

Thermo Scientific™ Pierce™ Formamide

Prepare hybridization cocktails and various denaturing solutions used with nucleic acids using this highly pure formulation of formamide denaturant.

Thermo Scientific™ 2X RNA Loading Dye Available on GSA/VA Contract for Federal Government customers only.

Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.

Thermo Scientific™ 3 M Sodium Acetate Solution, pH 5.2 Available on GSA/VA Contract for Federal Government customers only.

Precipitate DNA and RNA with this 0.22µm membrane-filtered, sterile, nuclease-free solution of sodium acetate.

Thermo Scientific™ 25 mM MgCl2 Available on GSA/VA Contract for Federal Government customers only.

Adjust magnesium concentrations to optimize your PCR and enzymatic reactions with Thermo Scientific™ 25mM MgCl2 solution.

Thermo Scientific™ X-Gluc

Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.

Thermo Scientific™ Pierce™ D-Luciferin

Firefly luciferase substrate for in vitro and in vivo applications

Thermo Scientific™ 6X MassRuler DNA Loading Dye Available on GSA/VA Contract for Federal Government customers only.

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.

Thermo Scientific™ Pierce™ Agarose

Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.

Thermo Scientific™ Pierce™ Tris/EDTA Solution

Consitently purify and store DNA without any worries using this highly purified, sterile, DNAse-free TE buffer (pH 8.0).

Thermo Scientific™ Pierce™ Glycerol

Perform a variety of protein and molecular biology methods using this highly purified glycerol (greater than 99% pure).

Thermo Scientific™ 0.5 M EDTA, pH 8.0 Available on GSA/VA Contract for Federal Government customers only.

Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.

Thermo Scientific Pierce™ D-Luciferin

Get convenience and high performance at a low cost in firefly luciferase reporter assays with our D-Luciferin, formulated at greater than 99% purity as both monosodium and monopotassium salts.

Thermo Scientific™ Pierce™ Ethidium Bromide

Visualize DNA fluorescently in a variety of molecular biology applications using these dilute (0.625mg/mL) or concentrated (10mg/mL) ethidium bromide solutions.

Thermo Scientific™ 6X Orange DNA Loading Dye Promotion

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels withThermo Scientific™ 6X Orange Loading Dye.

Thermo Scientific™ DEPC-treated Water Available on GSA/VA Contract for Federal Government customers only.

Optimize applications involving RNA with this deionized, diethylpyrocarbonate (DEPC) treated and 0.22µm membrane-filtered water.

Thermo Scientific™ 6X Orange DNA Loading Dye Available on GSA/VA Contract for Federal Government customers only.

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels withThermo Scientific™ 6X Orange Loading Dye.

Thermo Scientific™ X-Gal Solution, ready-to-use

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Thermo Scientific™ Pierce™ Tris

Perform a variety of protein and molecular biology methods using this standard Tris buffering reagent.

Thermo Scientific™ Pierce™ EDTA

Chelate alkaline earth metals such as iron, copper and zinc in a variety of laboratory methods using this highly purified EDTA.

Thermo Scientific™ 6X TriTrack DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X TriTrack DNA Loading Dye.

Thermo Scientific™ Nuclease-free Water Available on GSA/VA Contract for Federal Government customers only.

Optimize molecular biology applications with this deionized and 0.22µm membrane-filtered nuclease-free water.

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