Firefly Luciferase Vectors and Assays

Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Perform firefly luciferase assays in high-throughput screening applications.

Promega™ Bio-Glo™ Luciferase Assay System

Stable, inhibitor-resistant luciferase reagent for bioassays.

Promega™ Nano-Glo™ Dual-Luciferase™ Reporter Assay System

Ultra-sensitive detection of Firefly and NanoLuc™ luciferase activities in a single sample.

Promega™ ONE-Glo™ EX Luciferase Assay System

Highly sensitive assay with long-lived luminescence.

Thermo Scientific™ Red Firefly Luciferase Reporter Assay Vectors

Drive red-shifted firefly luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.

Promega™ Nano-Glo™ Dual-Luciferase™ Reporter Assay/pNL1.1.TK Bundle

Ultra-sensitive detection of Firefly and NanoLuc™ luciferase activities in a single sample. Improved firefly chemistry and the bright NanoLuc™ luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.

Promega™ pNL3.2.CMV Vector

Vector that expresses NanoLuc™ Luciferase using the CMV Promoter. The NlucP fusion protein serves as a negative control for experiments configured to measure changes in luciferase expression levels. This vector encodes a hygromycin resistance gene for selection in mammalian cells.

Promega™ pGL4.53[luc2/PGK] Vector

Vectors that are co-transfected into cells as controls in Dual-Reporter Assays. The PGK promoter-driven firefly control vectors should give an almost invariant level of activity, while the experimental Renilla vector varies with treatment.

Promega™ pGL2-Enhancer Vector

Vector carries the coding region for firefly (Photinus pyralis) luciferase, which is used to monitor transcriptional activity in transfected eukaryotic cells.

Promega™ pNL1.1.PGK[Nluc/PGK] Vector

Vectors containing NanoLuc™ Luciferase expressed from mammalian promoters. The pNL1.1.PGK[Nluc/PGK] Vectors are promoter-driven Nluc control vectors that can be co-transfected with experimental firefly luciferase vectors when using the NanoDLR™ Assay System.

Promega™ pGL4.54[luc2/TK] Vector

Vectors that are co-transfected into cells as controls in Dual-Reporter Assays. The TK promoter-driven firefly control vectors should give an almost invariant level of activity, while the experimental Renilla vector varies with treatment.

Promega™ pNLCoI1[luc2-P2A-NlucP/Hygro] Vector

Vectors that express both Firefly and NanoLuc™ luciferases from the same transcript, with no promoter. The pNLCoI Vectors are designed for use with the Nano-Glo™ Dual-Luciferase™ Reporter (NanoDLR™) Assay System.

Promega™ pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector

Vectors that express both Firefly and NanoLuc™ luciferases from the same transcript, with CMV promoter. The pNLCoI Vectors are designed for use with the Nano-Glo™ Dual-Luciferase™ Reporter (NanoDLR™) Assay System.

Promega™ pNL3.2[NlucP/minP] Vector

Clone response elements of interest to drive expression of NanoLuc™ Luciferase. NanoLuc™-PEST (NlucP) closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios.

Promega™ pFN11A (BIND) Flexi™ Vector

Designed for use with the CheckMate™/Flexi™ Vector Mammalian Two-Hybrid System (Mfr. No. C9360), the Flexi™ System, Entry/Transfer (Mfr. No. C8640) and the Flexi™ System, Transfer (Mfr. No. C8820).

Promega™ pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector

Vectors that express both Firefly and NanoLuc™ luciferases from the same transcript, with minimal promoter. The pNLCoI Vectors are designed for use with the Nano-Glo™ Dual-Luciferase™ Reporter (NanoDLR™) Assay System.

Promega™ pFN10A (ACT) Flexi™ Vector

Designed for use with the CheckMate™/Flexi™ Vector Mammalian Two-Hybrid System (Mfr. No. C9360), the Flexi™ System, Entry/Transfer (Mfr. No. C8640 ) and the Flexi™ System, Transfer (Mfr. No. C8820).

Promega™ pNL1.1.TK[Nluc/TK] Vector

Vectors containing NanoLuc™ Luciferase expressed from mammalian promoters. The pNL1.1.TK[Nluc/TK] Vectors are promoter-driven Nluc control vectors that can be co-transfected with experimental firefly luciferase vectors when using the NanoDLR™ Assay System.

Promega™ Nano-Glo™ Dual-Luciferase™ Reporter Assay/pGL4.53[luc2/PGK] Bundle

Ultra-sensitive detection of Firefly and NanoLuc™ luciferase activities in a single sample. Improved firefly chemistry and the bright NanoLuc™ luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.

Promega™ pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector

Vectors that express both Firefly and NanoLuc™ luciferases from the same transcript, with PGK promoter. The pNLCoI Vectors are designed for use with the Nano-Glo™ Dual-Luciferase™ Reporter (NanoDLR™) Assay System.

Promega™ pGL4.83[hRlucP/Puro] Vector

Cloning vectors for use as a Control Reporter Vector offering different reporter protein stabilities. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.

Promega™ Nano-Glo™ Dual-Luciferase™ Reporter Assay/pGL4.54[luc2/TK] Bundle

Ultra-sensitive detection of Firefly and NanoLuc™ luciferase activities in a single sample. Improved firefly chemistry and the bright NanoLuc™ luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.

Promega™ Nano-Glo™ Dual-Luciferase™ Reporter Assay/pNL1.1.PGK Bundle

Ultra-sensitive detection of Firefly and NanoLuc™ luciferase activities in a single sample. Improved firefly chemistry and the bright NanoLuc™ luciferase make this a highly sensitive dual assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.

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