Gaussia Luciferase Vectors and Assays

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.

Thermo Scientific™ Gaussia Luciferase Reporter Assay Vectors

Drive Gaussia luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

Promega™ Transfection Carrier DNA

Plasmid DNA used to dilute expression or reporter vectors for transfection. This product is made from pGEM™-3Zf(-) Vector but has been purified using a method that results in low endotoxin carryover, making it more suitable for use in transfecting mammalian cells.

Promega™ pNLF1-HIF1A [CMV/neo] Vector

Vectors to measure intracellular protein turnover of HIF1A. The HIF1A Vector System enables simple quantification of intracellular HIF1A protein levels to study the dynamics of this signaling protein in mediating cellular response to hypoxia.

Promega™ pNLF1-NRF2 [CMV/neo] Vector

Vectors to measure intracellular protein turnover of NRF2. The NRF2 Vector System enables simple quantification of intracellular NRF2 protein levels to study the dynamics of this signaling protein in mediating cellular response to oxidative stress.

Promega™ pFC32A Nluc CMV-Hygro Flexi™ Vector

Generate C-Terminal Fusions to NanoLuc™ Luciferase Reporter. The pFC32A and pFC32K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.

Promega™ pNLF1-secN [CMV/Hygro] Vector

Generate N-Terminal Fusions with N-Terminal Secreted NanoLuc&tradel Luciferase. The pNLF1 Vectors use traditional cloning with a multiple cloning site to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.

Promega™ pFN31K Nluc CMV-neo Flexi™ Vector

Generate N-Terminal fusions to NanoLuc™ Luciferase Reporter. The pFN31A and pFN31K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.

Promega™ pNLF1-C [CMV/Hygro] Vector

Generate C-Terminal Fusions with NanoLuc™ Enzyme. The pNLF1 Vectors use traditional cloning with a multiple cloning site (MCS) to generate C-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.

Promega™ pFN31A Nluc CMV-Hygro Flexi™ Vector

Generate N-Terminal fusions to NanoLuc™ Luciferase Reporter. The pFN31A and pFN31K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.

Promega™ pNLF1-N [CMV/Hygro] Vector

Generate N-Terminal Fusions with NanoLuc™ Enzyme. The pNLF1 Vectors use traditional cloning with a multiple cloning site (MCS) to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.

Promega™ pFC32K Nluc CMV-neo Flexi™ Vector

Generate C-Terminal Fusions to NanoLuc™ Luciferase Reporter. The pFC32A and pFC32K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.

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