Class Specific Protein Assay Kits
Promega™ ROS-Glo™ H2O2 Assay
Measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. Light signal that is proportional to the level of H2O2 present in the sample.
Promega™ ADP-Glo™ Max Assay
Produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats
Thermo Scientific™ Pierce™ Protease Assay Kits
Determine total protease activity in biological samples via fluorescent or colorimetric detection by measuring digestion of labeled casein substrate.
R&D Systems™ Malachite Green Phosphate Detection Kit
contains sufficient materials to run Sandwich ELISAs on at least 1 Kit
Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit
Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).
Promega™ DPPIV-Glo™ Protease Assay
Homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity
Boston Biochem™ 10X Ubiquitin Reconstitution Buffer
Formulated to provide the optimal formulation for the reconstitution and subsequent dilution of lyophilized Ubiquitin and Ubiquitin-like proteins
Boston Biochem™ 10X Ubiquitin Conjugation Reaction Buffer
Formulated to support the charging of Ubiquitin by E1 activating enzymes (the first reaction in the Ubiquitin conjugation cascade)
R&D Systems™ S-100 Fraction Degradation Kit, HeLa Cells
For the controlled degradation of exogenously added or endogenously contained substrate proteins through the ubiquitin proteasome pathway (UPP)
Boston Biochem™ 10X Energy Regeneration Solution
General Ubiquitin Proteasome Pathway (UPP) Products
Thermo Scientific™ Pierce™ Phosphoprotein Phosphate Estimation Kit
Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.
Boston Biochem™ 100X 20S Proteasome Activation Solution
Contains the optimal formulation for the chemical activation of all isoforms of constitutive 20S and immuno 20S proteasome enzymes including human (E-360), rabbit (E-350), rat (E-375), and mouse.
Boston Biochem™ 10X HSP90 Reaction Buffer
Formulated to support the in vitro enzymatic activity of Heat Shock Proteins, specifically HSP90 and the HSP90/p23 complex
R&D Systems™ S-100 Fraction Conjugation Kit, HeLa Cells
For the controlled conjugation of exogenously added or endogenously contained substrate proteins through the ubiquitin proteasome system (UPS)
Boston Biochem™ 10X HSP60 Reaction Buffer
Formulated to support the in vitro enzymatic activity of Heat Shock Proteins, specifically HSP60 and HSP60/HSP10 complex
Boston Biochem™ 10X SUMO Conjugation Reaction Buffer
Contains the optimal formulation for the use in conjugation assays of the ubiquitin-like modifiers, SUMO1 (UL-712), SUMO2 (UL-752), and SUMO3 (UL-762), to protein substrates in vitro.
Boston Biochem™ 10X NEDD8 Conjugation Reaction Buffer
Formulated to support in vitro conjugation assays with the Ubiquitin-like modifier NEDD8 (UL-812)
Boston Biochem™ 20X 20S Proteasome Reaction Buffer
Contains the optimal formulation for the quantitative analysis of constitutive 20S and immuno 20S proteasome activity
Zeptometrix OXItek Glutathione Reductase Assay Kit
Provides an indirect and highly reproducible method of quantifying the activity of total cellular glutathione reductase