Crosslinking, Labeling and Protein Modification

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution Available on GSA/VA Contract for Federal Government customers only.

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ DFDNB Crosslinker

Crosslink amines to amines via stable arylamine bonds using 1,5-difluoro-2,4-dinitrobenzene; an aryl halide compound with a short spacer arm (3.0A) and fluorine atoms.

Thermo Scientific™ Pierce™ PDPH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.

Thermo Scientific™ Pierce™ SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents Available on GSA/VA Contract for Federal Government customers only.

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Thermo Scientific™ Pierce™ Iodoacetic Acid Available on GSA/VA Contract for Federal Government customers only.

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ DyLight™ Fluor Phosphine Reagents

Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Available on GSA/VA Contract for Federal Government customers only.

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ EZ-Link™ Maleimide PEG11-Biotin

Biotinylate reduced cysteines with this long, maleimide-activated, sulfhydryl-reactive biotinylation reagent with an 11-unit PEG spacer arm to enhance solubility and reach.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ SPB Crosslinker

Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.

Thermo Scientific™ EZ-Link™ Hydrazide-Biocytin

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this short (19.7A) hydrazide-activated biotinylation reagent which also contains a second amine for conjugation.

Thermo Scientific™ Pierce™ SIA Crosslinker

Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.

Thermo Scientific™ Pierce™ Iodination Tubes

Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.

Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin

Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.

Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.

Thermo Scientific™ Pierce™ DTME Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.

Thermo Scientific™ Pierce™ Sulfo-LC-SPDP Crosslinker

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Thermo Scientific™ Pierce™ Biotin Quantitation Kit Available on GSA/VA Contract for Federal Government customers only.

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Pierce™ PMPI Crosslinker

Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.

Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker

Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).

Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer Reagent

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Thermo Scientific™ Pierce™ Azido-Sugars

Study glycoproteins using in vivo metabolic labeling and chemoselective ligation to phosphine reagents with these azide-tagged sugars (GlcNAz, GalNAz, ManNAz).

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits Available on GSA/VA Contract for Federal Government customers only.

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ Pierce™ Iodination Beads

Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction.

Thermo Scientific™ Pierce™ EDC Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

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