Crosslinking, Labeling and Protein Modification
Thermo Scientific™ DyLight™ Fluor Phosphine Reagents
Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ Pierce™ DMP Crosslinker
Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.
Thermo Scientific™ Pierce™ BS(PEG)n Crosslinkers
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Pierce™ Photoreactive Amino Acids
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ Pierce™ BMOE Crosslinker
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker
Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).
Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.
Thermo Scientific™ Pierce™ SPDP Crosslinker
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.
Thermo Scientific™ Pierce™ BMPH Crosslinker
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this short (8.1A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ Pierce™ Iodination Tubes
Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.
Thermo Scientific™ EZ-Link™ Hydrazide-LC-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (24.7A) hydrazide-activated biotinylation reagent.
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ SMCC Crosslinker
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Thermo Scientific™ EZ-Link™ Maleimide PEG11-Biotin
Biotinylate reduced cysteines with this long, maleimide-activated, sulfhydryl-reactive biotinylation reagent with an 11-unit PEG spacer arm to enhance solubility and reach.
Thermo Scientific™ Pierce™ PMPI Crosslinker
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.
Thermo Scientific™ Pierce™ SIA Crosslinker
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ SPB Crosslinker
Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.
Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ Sulfo-LC-SPDP Crosslinker
Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.
Thermo Scientific™ EZ-Link™ HPDP-Biotin
Biotinylate reduced protein or peptide cysteines with this pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent containing a cleavable disulfide bond.
Thermo Scientific™ EZ-Link™ Amine-PEG2-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this short (20.4A) biotinylation reagent containing a 2-unit polyethylene glycol spacer.
Thermo Scientific™ Pierce™ Iodination Reagent
Activate iodine for incorporation of iodine-125 labels into protein tyrosyl groups using this tetrachloro-diphenyl-glycoluril oxidizing agent.
Thermo Scientific™ Pierce™ Sulfo-SIAB Crosslinker
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.