Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt
Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ SMPB Crosslinker
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 10-atom (11.6A) spacer arm.
Thermo Scientific™ DyLight™ Near-IR Specialty Dyes
Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.
Thermo Scientific™ Pierce™ DTSSP Crosslinker
Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.
Thermo Scientific™ Pierce™ Carboxy-PEG-Amine Compounds
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ Pierce™ EDC Crosslinker
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ Pierce™ NHS-Phosphine Staudinger Ligation Reagents
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit
Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.
Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Thermo Scientific™ Pierce™ Immobilized Reductant Columns
Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits
Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin
Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.
Thermo Scientific™ DyLight™ Amine-Reactive Fluorescent Dyes
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
Thermo Scientific™ Pierce™ PEG4-SPDP Crosslinker
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ Pierce™ Biotin-PEG3-Phosphine
Label azide-tagged molecules for metabolic labeling (Staudinger ligation) and streptavidin detection using this phosphine-activated biotinylation reagent.
Thermo Scientific™ Pierce™ Methyl-PEG-Maleimide Reagents
Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).
Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (31.3A), pegylated biotinylation reagent containing a 4-unit PEG spacer for solubility.
Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Thermo Scientific™ Pierce™ Azido-Sugars
Study glycoproteins using in vivo metabolic labeling and chemoselective ligation to phosphine reagents with these azide-tagged sugars (GlcNAz, GalNAz, ManNAz).
Thermo Scientific™ DyLight™ In Vivo Apoptosis Detection Kits and Reagents
Measure drug-induced changes in apoptosis in vivo (live animal imaging) using near-infrared DyLight Dye fluorescent polycaspase probes.
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Pierce™ BSOCOES Crosslinker
Crosslink amines to amines with this crosslinker composed of NHS-ester groups and a sulfone-containing spacer arm (13.0A) that is base-cleavable.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits
Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.
Thermo Scientific™ DyLight™ Red Specialty Dyes
Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium-based, red-emitting (633 laser) NHS ester fluorescent dyes.
Thermo Scientific™ Pierce™ BS(PEG)n Crosslinkers
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Pierce™ DSS Crosslinker
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.