Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ EZ-Link™ Hydrazide-Biocytin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this short (19.7A) hydrazide-activated biotinylation reagent which also contains a second amine for conjugation.
Thermo Scientific™ Pierce™ PMPI Crosslinker
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.
Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ EZ-Link™ Amine-PEG2-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this short (20.4A) biotinylation reagent containing a 2-unit polyethylene glycol spacer.
Thermo Scientific™ Pierce™ Sulfo-SIAB Crosslinker
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.
Thermo Scientific™ Pierce™ Sulfo-LC-SPDP Crosslinker
Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.
Thermo Scientific™ DyLight™ Fluor Phosphine Reagents
Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.
Thermo Scientific™ Pierce™ MPBH Crosslinker
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (17.9A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ Pierce™ DTME Crosslinker
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientific™ Pierce™ DMP Crosslinker
Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.
Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker
Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).
Thermo Scientific™ Pierce™ N-Ethylmalemde (NEM)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin No-Weigh™ Format
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin No-Weigh™ Format is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Thermo Scientific™ Pierce™ Photoreactive Amino Acids
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ SMCC Crosslinker
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format
Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium cyclohexane spacer arm.
Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit
Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin No-Weigh™ Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin No-Weigh™ Format is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
BD 7-AAD Staining Solution
Convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI)
Thermo Scientific™ Pierce™ DSG Crosslinker
Crosslink amines to amines with disuccinimidyl glutarate with NHS-ester groups at the ends of a 7.7A spacer arm; membrane-permeable for intracellular protein crosslinking.
Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Pierce™ Traut's Reagent
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.