Crosslinking, Labeling and Protein Modification

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ No-Weigh™ Sulfo-SMCC

Format preserves reagent activity and prevents cross-contamination between uses

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits

Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ Pierce™ HABA Reagent

A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ Azido-Sugars

Study glycoproteins using in vivo metabolic labeling and chemoselective ligation to phosphine reagents with these azide-tagged sugars (GlcNAz, GalNAz, ManNAz).

Thermo Scientific™ DyLight™ Long Stokes Shift Specialty Dyes

Label antibodies or other proteins for FRET and multiplex fluorescence assays using fluors with large Stokes shifts (488nm excitation; yellow to far-red emission).

Thermo Scientific™ DyLight™ Amine-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP

Label primary antibodies with biotin for use in agarose or magnetic bead immunoprecipitation experiments using the Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP.

Thermo Scientific™ Pierce™ PEG4-SPDP Crosslinker

Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ EZ-Link™ Hydrazide-Biocytin

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this short (19.7A) hydrazide-activated biotinylation reagent which also contains a second amine for conjugation.

Thermo Scientific™ DyLight™ Blue Specialty Dye

Label amine-containing molecules for fluorescence imaging and flow cytometry with this coumarin-based, 405 laser-detectable, blue-emitting, NHS ester fluorescent dye.

Thermo Scientific™ Pierce™ AEDP Crosslinker

Crosslink amines to carboxyls with this spacer containing an internal disulfide bond; use EDC to modify functional groups on proteins and surfaces.

Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific™ DyLight™ Fluor Phosphine Reagents

Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.

Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ DyLight™ Near-IR Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.

Thermo Scientific™ Pierce™ PEG12-SPDP Crosslinker

Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ DyLight™ Quencher Specialty Dyes

Quench fluorescence resonance energy transfer (FRET) applications involving DyLight Fluors or other fluorescent probes using these non-fluorescent dyes.

Thermo Scientific™ Pierce™ PDPH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.

Thermo Scientific™ EZ-Link™ Biocytin

Synthesize long or trifunctional biotinylation reagents using this biotin variant containing a primary amine in its valeric acid chain.

Thermo Scientific Pierce™ Premium Grade Sulfo-NHS-LC-Biotin

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular amine-reactive biotinylation reagent.

Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit

Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement.

Thermo Scientific™ Pierce™ SM(PEG)n Crosslinkers

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific Pierce™ Premium Grade Sulfo-SMCC

Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.

Thermo Scientific™ EZ-Link™ PFP-Biotin

Biotinylate primary amines in proteins as well as secondary amines in DNA or RNA with this very short (9.6A) pentafluorophenyl-ester biotinylation reagent.

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