Crosslinking, Labeling and Protein Modification

Thermo Scientific™ DyLight™ Sulfhydryl-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin

Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.

Thermo Scientific™ EZ-Link™ Amine-PEG2-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this short (20.4A) biotinylation reagent containing a 2-unit polyethylene glycol spacer.

Thermo Scientific™ Pierce™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits

Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ Pierce™ BS3 (Sulfo-DSS) Crosslinker

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ DyLight™ Near-IR Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.

Thermo Scientific™ DyLight™ 680-4xPEG Amine-Reactive Dye and Kits

Fluorescently label antibodies and other proteins with Thermo Scientific™ DyLight 680-4xPEG Amine-Reactive Dye, a derivative of our high-performance DyLight 680 Dye.

Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.

Thermo Scientific™ DyLight™ Far Red Specialty Dyes

Label amine-containing molecules for fluorescence imaging and flow cytometry using these benzopyrillium-based, NHS ester, far red-emitting (647 laser) fluorescent dyes.

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Pierce™ NHS-Phosphine Staudinger Ligation Reagents

Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.

Thermo Scientific™ DyLight™ Fluor Antibody Labeling Kits

Label and purify antibodies for fluorescence imaging assays in one hour with the full spectral range of activated DyLight Fluorescent Dye kits.

Thermo Scientific™ POROS™ MabCapture™ A Select

MabCapture A Select a monoclonal antibody purification resin provides high dynamic binding at low linear flow rates and maintains superior binding capacity at high linear flow rates

Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this long (53.2A) biotinylation reagent containing an 11-unit polyethylene glycol spacer.

Thermo Scientific™ DyLight™ 800 4xPEG Maleimide

PEGylated NIR dye for enhanced fluorescence and reduced non-specific binding.

Thermo Scientific™ DyLight™ 550 2xPEG Maleimide

PEGylated dye for enhanced fluorescence and reduced non-specific binding.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.

Thermo Scientific™ Pierce™ Biotin Quantitation Kit

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin

Biotinylate protein amines and maximize solubility of proteins with this very long (56.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ Pierce™ Sulfo-SIAB Crosslinker

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.

Thermo Scientific Pierce™ Premium Grade Sulfo-NHS

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this N-hydroxysuccinimide modification reagent.

Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit

A column-based approach to antibody biotinylation and clean-up

Thermo Scientific™ DyLight™ Long Stokes Shift Specialty Dyes

Label antibodies or other proteins for FRET and multiplex fluorescence assays using fluors with large Stokes shifts (488nm excitation; yellow to far-red emission).

Thermo Scientific™ Pierce™ Photoreactive Amino Acids

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Thermo Scientific™ Pierce™ PMPI Crosslinker

Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ Pierce™ Biotin-PEG3-Phosphine

Label azide-tagged molecules for metabolic labeling (Staudinger ligation) and streptavidin detection using this phosphine-activated biotinylation reagent.

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