Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ TRITC Rhodamine Dyes and Labeling Kits
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.
Thermo Scientific™ DyLight™ Fluor Antibody Labeling Kits
Label and purify antibodies for fluorescence imaging assays in one hour with the full spectral range of activated DyLight Fluorescent Dye kits.
Thermo Scientific™ Pierce™ Methyl-PEG-Maleimide Reagents
Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).
Thermo Scientific™ DyLight™ In Vivo Apoptosis Detection Kits and Reagents
Measure drug-induced changes in apoptosis in vivo (live animal imaging) using near-infrared DyLight Dye fluorescent polycaspase probes.
Thermo Scientific™ Pierce™ SM(PEG)n Crosslinkers
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ Pierce™ Immobilized Reductant Columns
Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.
Thermo Scientific™ DyLight™ Near-IR Specialty Dyes
Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.
Thermo Scientific™ Pierce™ R-Phycoerythrin Fluorescent Protein
Create fluorescent probes (em 480, 545, 565; ex 578) using this red-fluorescent, multi-subunit protein that can be crosslinked or conjugated to other molecules.
Thermo Scientific™ Pierce™ EDC Crosslinker
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin and Labeling Kits
Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.
Thermo Scientific™ Pierce™ Azido-Sugars
Study glycoproteins using in vivo metabolic labeling and chemoselective ligation to phosphine reagents with these azide-tagged sugars (GlcNAz, GalNAz, ManNAz).
Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin
Biotinylate reduced cysteines with this short, maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a 2-unit PEG spacer arm to enhance water solubility.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific Pierce™ Premium Grade EDC
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular carbodiimide crosslinker.
Thermo Scientific™ Pierce™ PDPH Crosslinker
Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.
Thermo Scientific™ Pierce™ PEG12-SPDP Crosslinker
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits
Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.
Thermo Scientific™ Pierce™ Iodination Beads
Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction.
Thermo Scientific™ EZ-Link™ HPDP-Biotin
Biotinylate reduced protein or peptide cysteines with this pyridyldithiol-activated, sulfhydryl-reactive biotinylation reagent containing a cleavable disulfide bond.
Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt
Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.
Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer WB Kit
Tag a purified protein to covalently crosslink and transfer biotinylation to its binding partner; then detect the results by Western blotting.
Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ POROS™ MabCapture™ A Select
MabCapture A Select a monoclonal antibody purification resin provides high dynamic binding at low linear flow rates and maintains superior binding capacity at high linear flow rates
Thermo Scientific™ DyLight™ Fluor Phosphine Reagents
Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.
Thermo Scientific™ EZ-Link™ Maleimide PEG11-Biotin
Biotinylate reduced cysteines with this long, maleimide-activated, sulfhydryl-reactive biotinylation reagent with an 11-unit PEG spacer arm to enhance solubility and reach.