Crosslinking, Labeling and Protein Modification

Thermo Scientific™ DyLight™ Near-IR Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium- and cyanine-based, infrared-emitting (633 or 700 laser) NHS ester dyes.

Thermo Scientific™ Pierce™ Photoreactive Amino Acids Available on GSA/VA Contract for Federal Government customers only.

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Thermo Scientific™ DyLight™ Sulfhydryl-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Thermo Scientific™ DyLight™ Fluor Antibody Labeling Kits

Label and purify antibodies for fluorescence imaging assays in one hour with the full spectral range of activated DyLight Fluorescent Dye kits.

Thermo Scientific™ DyLight™ Blue Specialty Dye

Label amine-containing molecules for fluorescence imaging and flow cytometry with this coumarin-based, 405 laser-detectable, blue-emitting, NHS ester fluorescent dye.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Citraconic Anhydride

Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).

Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.

Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit

Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement.

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ Pierce™ SM(PEG)n Crosslinkers Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C) Available on GSA/VA Contract for Federal Government customers only.

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ Iodination Beads

Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction.

Thermo Scientific™ DyLight™ Green Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these rhodamine-based, 488 to 532 laser-detectable, green-emitting NHS ester fluorescent dyes.

Thermo Scientific™ Pierce™ EGS Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ DyLight™ Red Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these benzopyrillium-based, red-emitting (633 laser) NHS ester fluorescent dyes.

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin

Biotinylate protein amines and maximize solubility of proteins with this very long (56.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ EZ-Link™ NHS-LC-Biotin

Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.

Thermo Scientific™ Pierce™ EMCH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ DyLight™ Yellow Specialty Dyes

Label amine-containing molecules for fluorescence imaging or flow cytometry with these rhodamine-based, yellow-emitting (532 to 546 laser) NHS ester fluorescent dyes.

Thermo Scientific™ Pierce™ Sulfo-EGS Crosslinker

Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.

Thermo Scientific™ DyLight™ Far Red Specialty Dyes

Label amine-containing molecules for fluorescence imaging and flow cytometry using these benzopyrillium-based, NHS ester, far red-emitting (647 laser) fluorescent dyes.

Thermo Scientific™ Pierce™ HABA Reagent

A colored, biotin assay reagent HABA (4'-hydroxyazobenzene-2-carboxylic acid) to measure biotinylation levels of biotinylated antibodies or proteins.

Thermo Scientific™ DyLight™ In Vivo Apoptosis Detection Kits and Reagents

Measure drug-induced changes in apoptosis in vivo (live animal imaging) using near-infrared DyLight Dye fluorescent polycaspase probes.

Thermo Scientific™ DyLight™ Amine-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.

Thermo Scientific™ Pierce™ Proteinase K Available on GSA/VA Contract for Federal Government customers only.

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits Available on GSA/VA Contract for Federal Government customers only.

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

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