Crosslinking, Labeling and Protein Modification

Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ EZ-Link™ Hydrazide-LC-Biotin

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (24.7A) hydrazide-activated biotinylation reagent.

Thermo Scientific™ Pierce™ PMPI Crosslinker

Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin and Labeling Kits Available on GSA/VA Contract for Federal Government customers only.

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ Pierce™ BMPH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this short (8.1A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker

Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).

Thermo Scientific™ DyLight™ Fluor Phosphine Reagents

Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.

Thermo Scientific™ Pierce™ DTME Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.

Thermo Scientific™ Pierce™ Photoreactive Amino Acids Available on GSA/VA Contract for Federal Government customers only.

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Thermo Scientific™ Pierce™ Iodination Tubes

Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.

Thermo Scientific™ Pierce™ Biotin Quantitation Kit Available on GSA/VA Contract for Federal Government customers only.

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Pierce™ Iodoacetic Acid

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium cyclohexane spacer arm.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ SPDP Crosslinker

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.

Thermo Scientific™ Pierce™ Sulfo-SIAB Crosslinker

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution Available on GSA/VA Contract for Federal Government customers only.

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin

Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Thermo Scientific™ Pierce™ SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ EZ-Link™ Hydrazide-Biotin

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this simplest and shortest (15.7A) hydrazide-activated biotinylation reagent.

Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin

Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.

Thermo Scientific™ EZ-Link™ Biocytin

Synthesize long or trifunctional biotinylation reagents using this biotin variant containing a primary amine in its valeric acid chain.

Thermo Scientific™ Pierce™ DTBP Crosslinker

Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.

Thermo Scientific™ Pierce™ SATA

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

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