Crosslinking Reagents

Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker

Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).

Thermo Scientific™ Pierce™ PMPI Crosslinker

Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.

Thermo Scientific™ Pierce™ SIA Crosslinker

Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.

Thermo Scientific™ Pierce™ Sulfo-LC-SPDP Crosslinker

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Thermo Scientific™ Pierce™ SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents Available on GSA/VA Contract for Federal Government customers only.

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ PDPH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.

Thermo Scientific™ Pierce™ DFDNB Crosslinker

Crosslink amines to amines via stable arylamine bonds using 1,5-difluoro-2,4-dinitrobenzene; an aryl halide compound with a short spacer arm (3.0A) and fluorine atoms.

Thermo Scientific™ Pierce™ DTME Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.

Thermo Scientific™ Pierce™ SPB Crosslinker

Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.

Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer Reagent

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Thermo Scientific™ Pierce™ BMOE Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm.

Thermo Scientific™ Pierce™ DMS Crosslinker

Crosslink amines to amines with dimethyl suberimidate (CAS 34490-86-3); composed of imidoester groups at either end of a membrane-permeable, 8-atom spacer arm.

Thermo Scientific™ Pierce™ BS3 (Sulfo-DSS) Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers Available on GSA/VA Contract for Federal Government customers only.

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ EMCH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ EDC Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce™ SIAB Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom (10.6A) aminobenzoate spacer arm.

Thermo Scientific™ Pierce™ SPDP Crosslinker

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.

Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer WB Kit

Tag a purified protein to covalently crosslink and transfer biotinylation to its binding partner; then detect the results by Western blotting.

Thermo Scientific™ Pierce™ DMA Crosslinker

Crosslink amines to amines with dimethyl adipimidate (CAS 14620-72-5); composed of imidoester groups and a 6-atom, membrane-permeable spacer arm.

Thermo Scientific™ Pierce™ TMEA Crosslinker

A 3-way, sulfhydryl-to-sulfhydryl crosslinker; tris (2-maleimidoethyl) amine; composed of three maleimide groups on short spacer arms; conjugate protein or peptide cysteines.

Thermo Scientific™ Pierce™ PEG4-SPDP Crosslinker

Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce™ DSG Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to amines with disuccinimidyl glutarate with NHS-ester groups at the ends of a 7.7A spacer arm; membrane-permeable for intracellular protein crosslinking.

Thermo Scientific™ Pierce™ SBAP Crosslinker

Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and bromoacetyl (haloacetyl) groups at ends of a 7-atom (6.2A) spacer arm.

Thermo Scientific™ Pierce™ No-Weigh™ Sulfo-SMCC

Format preserves reagent activity and prevents cross-contamination between uses

Thermo Scientific™ Pierce™ DTBP Crosslinker

Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.

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