Protein Modification Reagents
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ Guanidine·HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ SATA
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format
Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ Pierce™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ Traut's Reagent
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ Pierce™ N-Ethylmalemde (NEM)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Pierce™ Hydroxylamine·HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific™ Pierce™ Immobilized Papain
Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Thermo Scientific™ Pierce™ Crystalline ACS Grade Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ Citraconic Anhydride
Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
Thermo Scientific™ Pierce™ MMTS
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)
MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.
Thermo Scientific™ Pierce™ SATP
Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ GlycoLink™ Coupling Catalyst
Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.
Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl
Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.
Thermo Scientific™ Pierce™ Proteinase K
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ Pierce™ Immobilized Reductant Columns
Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.