Protein Modification Reagents
Thermo Scientific™ Pierce™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ POROS™ MabCapture™ A Select
MabCapture A Select a monoclonal antibody purification resin provides high dynamic binding at low linear flow rates and maintains superior binding capacity at high linear flow rates
Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit
Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.
Thermo Scientific™ GlycoLink™ Coupling Catalyst
Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.
Thermo Scientific™ Pierce™ Citraconic Anhydride
Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Methyl-PEG-Amine Compounds
MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.
Thermo Scientific™ Pierce™ Carboxy-PEG-Amine Compounds
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ Pierce™ Factor Xa
Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.
Thermo Scientific™ Pierce™ Methyl-PEG-Maleimide Reagents
Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).
Thermo Scientific™ Pierce™ Immobilized Ficin
Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.
Thermo Scientific™ POROS™ MabCapture™ G Select
MabCapture G Select antibody affinity purification resin provides high dynamic binding at low linear flow rates and maintains superior binding capacity at high linear flow rates
Thermo Scientific™ Pierce™ Traut's Reagent
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin
Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.
Thermo Scientific™ WELQut Protease
Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ Pierce™ Immobilized Pepsin
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Carboxy-PEG-Thiol Compounds
CT(PEG)n: carboxylic acid and sulfhydryl compound with a 12-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver.
Thermo Scientific™ Pierce™ N-Ethylmalemde (NEM)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Pierce™ EMCA
Permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives) using this sulfhydryl-reactive (maleimide) compound.
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ POROS™ MabCapture™ A/G Select
MabCapture A/G Select allows antibody purification with high dynamic binding performance at low linear flow rates and maintains superior binding capacity at high linear flow rates
Thermo Scientific™ Pierce™ Crystalline ACS Grade Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ Cysteine·HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.
Thermo Scientific™ Pierce™ SATA
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ Proteinase K
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.