Protein Modification Reagents

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution Available on GSA/VA Contract for Federal Government customers only.

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Thermo Scientific™ Pierce™ N-Ethylmalemde (NEM) Available on GSA/VA Contract for Federal Government customers only.

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Traut's Reagent

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ TCEP·HCl Available on GSA/VA Contract for Federal Government customers only.

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format

Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ Pierce™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ SATA

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

Thermo Scientific™ Pierce™ Immobilized Papain Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Iodoacetic Acid

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Thermo Scientific™ Pierce™ Crystalline ACS Grade Sodium meta-Periodate

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).

Thermo Scientific™ Pierce™ MMTS

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel Available on GSA/VA Contract for Federal Government customers only.

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce™ Chloroacetamide No-Weigh™ Format

Thermo Scientific™ Pierce™ Chloroacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization.

Thermo Scientific™ Pierce™ Hydroxylamine·HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific Pierce™ TCEP-HCl, No-Weigh Format

Thermo Scientific™ Pierce™ TCEP-HCl is potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds.

EMD Millipore™ Proteinase K, Lyophilized Available on GSA/VA Contract for Federal Government customers only.

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

Thermo Scientific™ Pierce™ Citraconic Anhydride

Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ Pierce™ SATP

Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.

EMD Millipore™ Novagen™ Overnight Express™ Autoinduction System 1 Available on GSA/VA Contract for Federal Government customers only.

Induces protein expression without monitoring cell density.

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