Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ Immobilized Pepsin

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Proteinase K

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ Pierce™ Immobilized Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Thermo Scientific™ WELQut Protease

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

MP Biomedicals Pepsin

From porcine stomach mucosa

MP Biomedicals Trypsin-EDTA Solution

Concentration: 0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA (Versene)

EMD Millipore™ Proteinase K, Lyophilized Available on GSA/VA Contract for Federal Government customers only.

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

EMD Millipore™ Chymotrypsin, Human Pancreas

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

EMD Millipore™ Angiotensin Converting Enzyme-2, His•Tag™, Human, Recombinant, NSO Cells

Plays important role in regulating cardiovascular and renal functions

EMD Millipore™ Endopeptidase Lys-C, Achromobacter lyticus

Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues

EMD Millipore™ HRV 3C Protease

Highly efficient fusion tag removal at low temperature

EMD Millipore™ Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

Serine protease that specifically hydrolyzes peptide bonds at carboxylic side of glutamic acid and aspartic acid residues

EMD Millipore™ Dipeptidyl Peptidase IV (CD26), Porcine Kidney Available on GSA/VA Contract for Federal Government customers only.

Inhibition of DPPIV has been reported to be effective treatment for type II diabetes

EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

EMD Millipore™ Papain, Carica papaya Available on GSA/VA Contract for Federal Government customers only.

Cysteine protease that hydrolyzes peptides, amides and esters, especially at bonds involving basic amino acids, glycine or leucine

EMD Millipore™ Angiotensin Converting Enzyme, His•Tag™, Human, Recombinant, NSO Cells

Type I membrane protein that exists in two isoforms, somatic and germinal