Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ WELQut Protease

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Thermo Scientific™ Pierce™ Proteinase K

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ NeuCode™ Lysine-080 Protein Sample Preparation Kit

Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.

Thermo Scientific™ Pierce™ Immobilized Pepsin

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Thermo Scientific™ Pierce™ Immobilized Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

MP Biomedicals Pepsin

From porcine stomach mucosa

MP Biomedicals Trypsin-EDTA Solution

Concentration: 0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA (Versene)

EMD Millipore™ Papain, Carica papaya Available on GSA/VA Contract for Federal Government customers only.

Cysteine protease that hydrolyzes peptides, amides and esters, especially at bonds involving basic amino acids, glycine or leucine

EMD Millipore™ Thrombin, Restriction Grade

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

EMD Millipore™ Dipeptidylpeptidase IV, His•Tag™, Human, Recombinant, S. frugiperda

Inhibition of DPPIV has been reported to be effective treatment for type II diabetes

EMD Millipore™ Proteinase K Solution, 600mAU/mL Available on GSA/VA Contract for Federal Government customers only.

Efficient removal of proteins from nucleic acid solutions

EMD Millipore™ S•Tag Thrombin Purification Kit Available on GSA/VA Contract for Federal Government customers only.

Application in protein purification

EMD Millipore™ Dipeptidyl Peptidase IV (CD26), Porcine Kidney Available on GSA/VA Contract for Federal Government customers only.

Inhibition of DPPIV has been reported to be effective treatment for type II diabetes

EMD Millipore™ Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins

EMD Millipore™ Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

Serine protease that specifically hydrolyzes peptide bonds at carboxylic side of glutamic acid and aspartic acid residues

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