Proteases and Protein-Cleaving Reagents
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ Proteinase K
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Immobilized Pepsin
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set
TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.
Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit
Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)
MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.
Thermo Scientific™ Pierce™ Immobilized Ficin
Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Immobilized Papain
Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt
Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.
Thermo Scientific™ Pierce™ TPCK Trypsin and Resin
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
EMD Millipore™ Thrombin, Restriction Grade
Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV
Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit
Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.
Promega™ ProTEV Plus Protease
Engineered protease with greater stability and prolonged activity over native TEV protease.
EMD Millipore™ Proteinase K Solution, 600mAU/mL
Efficient removal of proteins from nucleic acid solutions
MP Biomedicals™ Trypsin-EDTA Solution
widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.
EMD Millipore™ Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides
MP Biomedicals™ Trypsin, Porcine Pancreas
Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
EMD Millipore™ Endopeptidase Lys-C, Achromobacter lyticus
Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues