Proteases and Protein-Cleaving Reagents
Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin
Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Immobilized Pepsin
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Proteinase K
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ TPCK Trypsin and Resin
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt
Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.
Thermo Scientific™ Pierce™ Immobilized Papain
Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit
Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit
Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.
Thermo Scientific™ Pierce™ Factor Xa
Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.
Thermo Scientific™ Pierce™ Immobilized Ficin
Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.
Thermo Scientific™ WELQut Protease
Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.
MP Biomedicals Trypsin-EDTA Solution
Concentration: 0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA (Versene)
EMD Millipore™ Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
EMD Millipore™ Chymotrypsin, Human Pancreas
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV
EMD Millipore™ Angiotensin Converting Enzyme-2, His•Tag™, Human, Recombinant, NSO Cells
Plays important role in regulating cardiovascular and renal functions
EMD Millipore™ Endopeptidase Lys-C, Achromobacter lyticus
Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues
EMD Millipore™ Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
Serine protease that specifically hydrolyzes peptide bonds at carboxylic side of glutamic acid and aspartic acid residues
EMD Millipore™ Dipeptidyl Peptidase IV (CD26), Porcine Kidney
Inhibition of DPPIV has been reported to be effective treatment for type II diabetes
EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides
EMD Millipore™ Papain, Carica papaya
Cysteine protease that hydrolyzes peptides, amides and esters, especially at bonds involving basic amino acids, glycine or leucine