Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Proteinase K Available on GSA/VA Contract for Federal Government customers only.

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C) Available on GSA/VA Contract for Federal Government customers only.

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ Immobilized Pepsin Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit Available on GSA/VA Contract for Federal Government customers only.

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Promega™ IdeS Protease

Antibody fragmentation and characterization, simplified.

EMD Millipore™ HRV 3C Protease Available on GSA/VA Contract for Federal Government customers only.

Highly efficient fusion tag removal at low temperature

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

MP Biomedicals™ Pepsin

From porcine stomach mucosa

Thermo Scientific™ Pierce™ Immobilized Papain Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt Available on GSA/VA Contract for Federal Government customers only.

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

EMD Millipore™ Thrombin, Restriction Grade Available on GSA/VA Contract for Federal Government customers only.

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Promega™ ProTEV Plus Protease

Engineered protease with greater stability and prolonged activity over native TEV protease.

Affymetrix/USB™ Pepsin from Porcine Stomach

Derived from Porcine stomach

EMD Millipore™ Proteinase K Solution, 600mAU/mL Available on GSA/VA Contract for Federal Government customers only.

Efficient removal of proteins from nucleic acid solutions

MP Biomedicals™ Trypsin-EDTA Solution

widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

EMD Millipore™ Proteinase K, Lyophilized Available on GSA/VA Contract for Federal Government customers only.

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

Trypsin, porcine pancreas

A pancreatic serine protease

EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

Trypsin, bovine pancreas

A pancreatic serine protease

MP Biomedicals™ Trypsin, Porcine Pancreas

Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Papain, Carica papaya Latex

A cysteine protease; breaks down intercellular matrix of cartilage

EMD Millipore™ Endopeptidase Lys-C, Achromobacter lyticus Available on GSA/VA Contract for Federal Government customers only.

Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues

Pepstatin A, 98%

Protease inhibitor that acts on pepsin, renin and cathepsin D

  spinner