Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Promega™ IdeS Protease

Antibody fragmentation and characterization, simplified.

Thermo Scientific™ Pierce™ Immobilized Papain Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

MP Biomedicals™ Pepsin

From porcine stomach mucosa

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Thermo Scientific™ Pierce™ Proteinase K Available on GSA/VA Contract for Federal Government customers only.

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

EMD Millipore™ HRV 3C Protease Available on GSA/VA Contract for Federal Government customers only.

Highly efficient fusion tag removal at low temperature

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ Pierce™ Immobilized Pepsin Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C) Available on GSA/VA Contract for Federal Government customers only.

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Promega™ ProTEV Plus Protease

Engineered protease with greater stability and prolonged activity over native TEV protease.

Promega AccuMAP Low pH Protein Digestion Mini Kit, 1 each

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit Available on GSA/VA Contract for Federal Government customers only.

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Promega Caspase-Glo 1 Inflammasome Assay, 10ml

The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.

Promega Caspase-Glo 1 Inflammasome Assay, 5 x 10ml

The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.

Promega AccuMAP Low pH Resistant rLys-C Solution, 300microl

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

EMD Millipore™ Thrombin, Restriction Grade Available on GSA/VA Contract for Federal Government customers only.

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt Available on GSA/VA Contract for Federal Government customers only.

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

Pepstatin A, 98%

Protease inhibitor that acts on pepsin, renin and cathepsin D

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Thermo Scientific™ WELQut Protease

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

EMD Millipore™ Proteinase K, Lyophilized Available on GSA/VA Contract for Federal Government customers only.

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

EMD Millipore™ Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi Available on GSA/VA Contract for Federal Government customers only.

Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins

Trypsin, porcine pancreas

A pancreatic serine protease

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