Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Immobilized Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Thermo Scientific™ TMTsixplex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific™ Pierce™ Immobilized Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Proteinase K

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ Pierce™ Immobilized Pepsin

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ NeuCode™ Lysine-080 Protein Sample Preparation Kit

Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering.

Thermo Scientific™ WELQut Protease

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ NeuCode™ Lysine-080 Protein Sample Preparation Kit

Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.

Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ TPCK Trypsin and Resin

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

MP Biomedicals Trypsin-EDTA Solution

Concentration: 0.05% (w/v) Trypsin (1:250) and 0.02% (w/v) EDTA (Versene)

MP Biomedicals Pepsin

From porcine stomach mucosa

EMD Millipore™ Dipeptidylpeptidase IV, His•Tag™, Human, Recombinant, S. frugiperda

Inhibition of DPPIV has been reported to be effective treatment for type II diabetes

EMD Millipore™ Proteinase K Solution, 600mAU/mL Available on GSA/VA Contract for Federal Government customers only.

Efficient removal of proteins from nucleic acid solutions

EMD Millipore™ Dipeptidyl Peptidase IV (CD26), Porcine Kidney Available on GSA/VA Contract for Federal Government customers only.

Inhibition of DPPIV has been reported to be effective treatment for type II diabetes

EMD Millipore™ β-Lactamase, Bacillus cereus 569/H9

Useful for assaying penicillins and cephalosporins

EMD Millipore™ Calbiochem™ MMP-2/MMP-7 Substrate, Fluorogenic

Extremely sensitive fluorogenic substrate for matrix metalloproteinases (MMPs)

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