Proteases and Protein-Cleaving Reagents
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ Immobilized Papain
Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Immobilized Pepsin
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Proteinase K
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)
MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.
Thermo Scientific™ Pierce™ Human Rinovirus 3C Protease (HRV 3C)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Immobilized Ficin
Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit
Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.
Promega™ ProTEV Plus Protease
Engineered protease with greater stability and prolonged activity over native TEV protease.
Thermo Scientific™ Pierce™ TPCK Trypsin and Resin
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
Thermo Scientific™ Pierce™ In-Gel Tryptic Digestion Kit
Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
Promega Caspase-Glo 1 Inflammasome Assay, 10ml
The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.
EMD Millipore™ Thrombin, Restriction Grade
Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV
EMD Millipore™ Proteinase K Solution, 600mAU/mL
Efficient removal of proteins from nucleic acid solutions
EMD Millipore™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides
EMD Millipore™ Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
Promega AccuMAP Low pH Resistant rLys-C Solution, 300microl
AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.
EMD Millipore™ Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins
EMD Millipore™ Endopeptidase Lys-C, Achromobacter lyticus
Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues