Reducing Agents for Protein Disulfides

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

Thermo Scientific™ Pierce™ Immobilized Reductant Columns

Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

EMD Millipore™ Calbiochem TCEP, Neutral Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use stable, neutral form of popular disulfide-reducing agent TCEP, Hydrochloride

EMD Millipore™ Calbiochem™ SPUTOLYSIN™ Reagent Available on GSA/VA Contract for Federal Government customers only.

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

EMD Millipore™ Calbiochem™ TPEN

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes

EMD Millipore™ Calbiochem™ PIH

Cell-permeable, non-toxic tridendate iron (Fe3+) chelator of aroyl hydrazone class

EMD Millipore™ Calbiochem™ BAPTA/AM Available on GSA/VA Contract for Federal Government customers only.

Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA

EMD Millipore™ Calbiochem™ TCEP, Hydrochloride

Reduces even the most stable water-soluble alkyl disulfides at pH 4.5 within 5 minutes

EMD Millipore™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis

EMD Millipore™ Calbiochem EGTA/AM Available on GSA/VA Contract for Federal Government customers only.

Application in Life Science research