Reducing Agents for Protein Disulfides

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Pierce™ Immobilized Reductant Columns

Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

EMD Millipore™ Calbiochem™ TPEN

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes

EMD Millipore™ Calbiochem™ SPUTOLYSIN™ Reagent

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

EMD Millipore™ Calbiochem™ TCEP, Hydrochloride

Reduces even the most stable water-soluble alkyl disulfides at pH 4.5 within 5 minutes

EMD Millipore™ Calbiochem EGTA/AM

Application in Life Science research

EMD Millipore™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis

EMD Millipore™ Calbiochem™ BAPTA/AM

Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA

EMD Millipore™ Calbiochem TCEP, Neutral

Ready-to-use stable, neutral form of popular disulfide-reducing agent TCEP, Hydrochloride

EMD Millipore™ Calbiochem™ PIH

Cell-permeable, non-toxic tridendate iron (Fe3+) chelator of aroyl hydrazone class