Reducing Agents for Protein Disulfides
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl
Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
Thermo Scientific™ Pierce™ Cysteine·HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.
Thermo Scientific Pierce™ TCEP-HCl, No-Weigh Format
Thermo Scientific™ Pierce™ TCEP-HCl is potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds.
Thermo Scientific™ Pierce™ Immobilized Reductant Columns
Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.
EMD Millipore™ Calbiochem™ TCEP, Hydrochloride
Reduces even the most stable water-soluble alkyl disulfides at pH 4.5 within 5 minutes
EMD Millipore™ Calbiochem™ EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Suitable for use in molecular biology applications
EMD Millipore™ Calbiochem TCEP, Neutral
Ready-to-use stable, neutral form of popular disulfide-reducing agent TCEP, Hydrochloride
EMD Millipore™ Calbiochem™ PIH
Cell-permeable, non-toxic tridendate iron (Fe3+) chelator of aroyl hydrazone class
EMD Millipore™ Calbiochem™ EDTA, 500mM Solution, pH 8.0, ULTROL™ Grade
Application in Life Science research
EMD Millipore™ Calbiochem™ TPEN
Lipid-soluble heavy metal chelator that crosses artificial and natural membranes
EMD Millipore™ Calbiochem™ SPUTOLYSIN™ Reagent
Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum
EMD Millipore™ Calbiochem™ Cleland’s Reagent, Molecular Biology Grade
Protective agent for SH groups
EMD Millipore™ Calbiochem™ BAPTA/AM
Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA
EMD Millipore™ Calbiochem™ DM-NITROPHEN™ Reagent, Tetrasodium Salt
Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis