Immunohistochemistry Kits and Reagents

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules Available on GSA/VA Contract for Federal Government customers only.

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain Available on GSA/VA Contract for Federal Government customers only.

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Thermo Scientific™ Pierce™ Immunostain Enhancer

Increase signal intensity 3- to 12-fold and antibody dilution 5- to 20-fold beyond normal using both chromogenic or fluorescent IHC substrates.

Thermo Scientific™ Pierce™ ABC Staining Kits Available on GSA/VA Contract for Federal Government customers only.

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain Available on GSA/VA Contract for Federal Government customers only.

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce™ Protein Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Bundles the Pierce Metal Enhanced DAB Substrate Kit with all components to stain, counterstain and preserve results from frozen or paraffin tissue sections.

Thermo Scientific™ Pierce™ beta-Galactosidase Precipitating Substrates

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

Thermo Scientific™ Pierce™ Peroxidase Suppressor

Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.

BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E

M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by I-A[b], I-A[d], I-A[q], I-E[d], I-E[k] MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice.

BD Horizon™ BV480 Mouse Anti-Human CD8

CD8 is expressed by majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. BD Horizon™ BV480 Mouse Anti-Human CD8 plays important roles in T cell activation and selection.

BD Cytometric Bead Array (CBA) Human IgA; Flex Set, Bead C9 Available on GSA/VA Contract for Federal Government customers only.

Bead-based immunoassay for measuring human total immunoglobulin A (IgA) in serum and plasma samples

BD Horizon™ BV480 Rat Anti-Mouse IgD

The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes and it does not react with other immunoglobulin isotypes. BD Horizon™ BV480 Rat Anti-Mouse IgD is expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro.

BD Cytometric Bead Array (CBA) Phospho STAT1 (Y701); Flex Set, Bead C5 Available on GSA/VA Contract for Federal Government customers only.

Bead-based immunoassay for measuring human or mouse signal transducer and activator of transcription 1 (Stat1) that has been tyrosine-phosphorylated at Tyr-701 in denatured cell lysate samples

BD Horizon™ BV480 Mouse Anti-Human CD19

CD19 is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Mouse Anti-Human CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD Cytometric Bead Array (CBA) Human IgE; Flex Set, Bead E9 Available on GSA/VA Contract for Federal Government customers only.

Bead-based immunoassay for measuring human total immunoglobulin E (IgE) in serum and plasma samples

BD Horizon™ BV480 Mouse Anti-Rat CD90/Mouse CD90.1

CD90 (Thy-1) is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. BD Horizon™ BV480 Mouse Anti-Rat CD90/Mouse CD90.1 also mediates adhesion of mouse thymocytes to mouse thymic stroma. The OX-7 clone has also been reported to crossreact with rabbit and guinea pig thymus, brain, and intestine.

BD Cytometric Bead Array (CBA) Human IL-17A; Flex Set, Bead B5 Available on GSA/VA Contract for Federal Government customers only.

Determines human reactivity by testing samples with BD CBA Human IL-13 Flex Set

BD Horizon™ BV480 Rat Anti-Mouse CD19

Expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Rat Anti-Mouse CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD Cytometric Bead Array (CBA) Rat IL-2 Enhanced Sensitivity Flex Set (Bead E5) Available on GSA/VA Contract for Federal Government customers only.

Measures rat IL-2 in serum and cell culture supernatant samples

BD Human IL-12 (p70) ELISA Kit Available on GSA/VA Contract for Federal Government customers only.

For the in vitro quantitative determination of human IL-12 (p70) in serum, plasma, and cell culture supernatant

BD Horizon™ BV480 Mouse Anti-Human CD20

CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. BD Horizon™ BV480 Mouse Anti-Human CD20 is observed in low levels on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.

BD Horizon™ BV480 Mouse Anti-Human CD4

CD4 is expressed on T-helper/inducer lymphocytes and monocytes. BD Horizon™ BV480 Mouse Anti-Human CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

BD ELISPOT Sets Available on GSA/VA Contract for Federal Government customers only.

Sufficient capture antibody, detection antibody, and streptavidin-horseradish peroxidase for 10 × 96 wells

BD Cytometric Bead Array (CBA) Mouse CD62E (E-Selectin); Flex Set, Bead E6 Available on GSA/VA Contract for Federal Government customers only.

Bead-based immunoassay capable of measuring mouse soluble CD62E (E-Selectin) in cell culture supernatant samples

BD Horizon™ BV480 Mouse Anti-Human CD16

Expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. BD Horizon™ BV480 Mouse Anti-Human CD16 clone 3G8 also cross-reacts with a  subset of peripheral blood lymphocytes and monocytes, but not granulocytes, of baboon and both rhesus and cynomologus macaque monkeys.

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