Amino Acid Analysis Reagents and Accessories
Thermo Scientific™ Pierce™ Fluoraldehyde (OPA) Reagent
Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).
Thermo Scientific™ Pierce™ p-Bromophenacylate Reagent
Label, detect and measure peptides and amino acids by HPLC using this carboxylic acid-reactive compound (Amax 260nm; UV); also called p-Bromophenacyl-8.
Thermo Scientific Pierce™ Ninhydrin
Label, detect and measure peptides, proteins and amino acids by HPLC (liquid chromatography) using this amine-reactive, colored reagent (Amax 440nm).
Thermo Scientific™ Pierce™ Vacuum Hydrolysis Tubes
High-quality valved glass tubes designed for heated, vacuum, acid-hydrolysis of proteins and peptides for amino acid analysis by HPLC chromatography.
Thermo Scientific™ Pierce™ Peptide Retention Standard
A mixture of five specific deca-peptides (acetylated with c-terminal amides) to run reference elution profiles for reverse-phase HPLC chromatography.
Thermo Scientific™ Pierce™ TNBSA (Trinitrobenzene Sulfonic Acid)
Label primary amines (peptides or amino acids) for colorimetric detection (Amax 335nm) using this 5% solution of trinitrobenzene sulfonic acid in methanol.
Thermo Scientific™ Pierce™ Fluoraldehyde (OPA) Crystals
Measure peptides or amino acids (Ex 340nm, Em 455nm) with phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent.
Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Pierce™ FDAA (Marfey's Reagent)
Label and measure optical isomers of amino acids by reverse-phase chromatography (HPLC) with this amine-reactive, colored (Amax 340nm) compound.
Thermo Scientific™ Pierce™ PITC (Edman's Reagent)
Phenylisothiocyanate, an amine-reactive, colored compound (Amax 254nm; UV) for pre-column derivatization of amino acids for reverse-phase HPLC.
Thermo Scientific™ Pierce™ Amino Acid Standard H
Calibrate HPLC analysis of protein hydrolysates using this calibration standard mixture of 18 amino acids, each at 2.5 micromoles/mL in 0.1N HCl.
Thermo Scientific™ Pierce™ Hydrochloric Acid Solution
Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.