Protein Extraction and Purification

eBioscience™ Protein Transport Inhibitor Cocktail, 500x Non-distribution product as customer accommodation.

Addition to cells in culture inhibits intracellular protein secretory/transport pathway, resulting in accumulation of secreted proteins in the lumen of the ER and in the Golgi apparatus.

Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits Available on GSA/VA Contract for Federal Government customers only.

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ HisPur™ Ni-NTA Resin Available on GSA/VA Contract for Federal Government customers only.

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates Available on GSA/VA Contract for Federal Government customers only.

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ ActivX™ Fluorophosphonate Probes

Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

Thermo Scientific™ Pierce™ Streptavidin Resins Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ Protein A/G Agarose and Kits Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with versatile, recombinant Protein A/G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Magnetic ChIP Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Thermo Scientific™ GlycoLink™ Immobilization Kits and Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Invitrogen™ Molecular Probes™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution

Thermo Scientific™ Pierce™ Classic Magnetic IP and Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Easily immunoprecipitate target protein complexes by magnetic separation using high-quality Protein A/G magnetic beads and optimized buffers.

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Thermo Scientific™ Pierce™ RIPA Buffer Available on GSA/VA Contract for Federal Government customers only.

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce™ NHS-Activated Agarose

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ Pierce™ HA Tag IP/Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Thermo Scientific™ Pierce™ High-Capacity Protein L Agarose

Purify antibodies containing kappa light chains or perform immunoprecipitation with this high-capacity Protein L resin on beaded agarose.

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution Available on GSA/VA Contract for Federal Government customers only.

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Invitrogen™ Total Exosome Isolation Reagent (from cell culture media) Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Easily isolate exosomes and avoid time-consuming ultra-centrifugation

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Protein L Agarose and Kits

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Agarose ChIP Kit Available on GSA/VA Contract for Federal Government customers only.

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits Available on GSA/VA Contract for Federal Government customers only.

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ Protein AG Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis Available on GSA/VA Contract for Federal Government customers only.

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

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