Protein Extraction and Purification

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution Available on GSA/VA Contract for Federal Government customers only.

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Streptavidin Resins Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits Available on GSA/VA Contract for Federal Government customers only.

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit Available on GSA/VA Contract for Federal Government customers only.

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Thermo Scientific™ Pierce™ Protein L Agarose and Kits

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ ActivX™ Fluorophosphonate Probes

Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

Thermo Scientific™ AminoLink™ Plus Coupling Resin and Immobilization Kits Available on GSA/VA Contract for Federal Government customers only.

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit Available on GSA/VA Contract for Federal Government customers only.

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Thermo Scientific™ Pierce™ RIPA Buffer Available on GSA/VA Contract for Federal Government customers only.

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits Available on GSA/VA Contract for Federal Government customers only.

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific™ Pierce™ HA Tag IP/Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit Available on GSA/VA Contract for Federal Government customers only.

Covalently immobilize IP antibodies and perform co-IP assays in which target protein complexes are immunoprecipitated without antibody contamination.

Thermo Scientific™ GlycoLink™ Immobilization Kits and Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Thermo Scientific™ Pierce™ Magnetic ChIP Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Thermo Scientific™ Pierce™ High-Capacity Protein A/G Agarose Available on GSA/VA Contract for Federal Government customers only.

Purify antibodies or perform immunoprecipitation with this exceptionally high-capacity, recombinant Protein A/G beaded agarose.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates Available on GSA/VA Contract for Federal Government customers only.

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ Pierce™ Protein Refolding Kit

Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ HisPur™ Ni-NTA Resin Available on GSA/VA Contract for Federal Government customers only.

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ CarboxyLink™ Coupling Resins and Immobilization Kits

Covalently immobilize peptide antigens and other carboxylate ligands for affinity purification using DADPA-activated agarose beads and EDC crosslinker.

Thermo Scientific™ Pierce™ Protein A/G Agarose and Kits Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with versatile, recombinant Protein A/G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Magnetic RNA-Protein Pull-Down Kit Available on GSA/VA Contract for Federal Government customers only.

Utilize a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Anti-c-Myc Agarose Available on GSA/VA Contract for Federal Government customers only.

Highly specific resin for recombinant c-Myc-tagged protein purification.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin Available on GSA/VA Contract for Federal Government customers only.

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ Pierce™ High-Capacity Protein L Agarose

Purify antibodies containing kappa light chains or perform immunoprecipitation with this high-capacity Protein L resin on beaded agarose.

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