Protein Extraction and Purification

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits Available on GSA/VA Contract for Federal Government customers only.

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ IgG Plus Orientation Kits Available on GSA/VA Contract for Federal Government customers only.

Bind and covalently immobilize antibodies to create a reusable affinity purification column with high binding capacity with Protein A or G agarose columns and DSS crosslinker.

Alfa Aesar™ Lysis Buffer, pH 8.0

For isolation of His-tag modified proteins

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Thermo Scientific™ Surfact-Amps™ Purified Detergent Sampler

Convenient collection of various Surfact-Amps Detergent Solutions and other selected useful detergents for protein research methods.

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

Thermo Scientific™ AminoLink™ Plus Coupling Resin and Immobilization Kits Available on GSA/VA Contract for Federal Government customers only.

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Thermo Scientific™ Pierce™ Magnetic ChIP Kit Available on GSA/VA Contract for Federal Government customers only.

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

Thermo Scientific™ HiPPR Detergent Removal Resin and Kits Available on GSA/VA Contract for Federal Government customers only.

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

Thermo Scientific™ Pierce™ High-Capacity Protein A Agarose and Kits Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ CarboxyLink™ Coupling Resins and Immobilization Kits

Covalently immobilize peptide antigens and other carboxylate ligands for affinity purification using DADPA-activated agarose beads and EDC crosslinker.

Thermo Scientific™ Pierce™ HA Tag IP/Co-IP Kit Available on GSA/VA Contract for Federal Government customers only.

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Alfa Aesar™ Phosphatase Inhibitor Cocktail IV

For inhibition of both serine/threonine and alkaline phosphatases

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Thermo Scientific™ Pierce™ Protein G Agarose and Kits Available on GSA/VA Contract for Federal Government customers only.

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Disposable Plastic Columns Available on GSA/VA Contract for Federal Government customers only.

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ HisPur™ Cobalt Superflow Agarose Available on GSA/VA Contract for Federal Government customers only.

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Thermo Scientific™ Pierce™ DNase I Available on GSA/VA Contract for Federal Government customers only.

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Pierce™ Monomeric Avidin Resins Available on GSA/VA Contract for Federal Government customers only.

Reversibly bind biotinylated proteins and other ligands with monomers of avidin covalently attached as individual subunits onto beaded resins.

Thermo Fisher Scientific™ Pierce™ Glutathione Agarose

Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate 

Thermo Scientific™ Pierce™ Kinase Enrichment Kits and Probes

Desthiobiotin-ATP and -ADP probes to label, affinity purify and measure the active site of ATPases (kinases), including chaperones and metabolic enzymes.

Alfa Aesar™ Factor XA Protease Digestion Buffer

For His-tag protein purification from mammalian cells

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