Protein Extraction and Purification

Corning™ Spin-X™ UF Concentrators Available on GSA/VA Contract for Federal Government customers only.

Disposable, single use only ultrafiltration devices with polyethersulfone membranes (PES) for the centrifugal concentration and/or purification of biological fluids and aqueous solutions

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

R&D Systems Glycosyltransferase Activity Kit

A simple, nonradioactive, high throughput-compatible format for assaying the enzyme activity of glycosyltransferases that use diphosphonucleotide sugars as donor substrates

Thermo Scientific™ Pierce™ n-Dodecyl-β-Maltoside

Water-soluble, non-denaturing and nonionic detergent especially useful for solubilizing hydrophobic membrane proteins to preserve their activity.

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Thermo Scientific™ HiPPR Detergent Removal Resin and Kits

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

Thermo Scientific™ Pierce™ Sodium Dodecyl Sulfate (SDS) Lauryl

Perform routine protein electrophoresis and cell lysis methods using this popular, standard-grade SDS detergent.

R&D Systems Rat CD8+ T Cell Enrichment Columns

Designed to prepare sub-populations of CD8+ rat T cells via high affinity negative selection

Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis

Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Thermo Scientific™ UltraLink™ Biosupport

Covalently immobilize proteins and other primary amine biomolecules for affinity purification with this durable, azlactone-activated polyacrylamide resin.

R&D Systems Leupeptin, CF

A general, reversible cystein protease inhibitor and trypsin-like serine protease inhibitor

Thermo Scientific™ Pierce™ Protein A/G Agarose and Kits

Affinity purify antibodies (IgG) with versatile, recombinant Protein A/G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Sodium Dodecyl Sulfate, 20% Solution (Electrophoresis/Molecular Biology), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: Sodium Dodecyl Sulfate Name Note: 20% Solution CAS: 7732-18-5 CAS: 151-21-3 Purity Grade: Electrophoresis/Molecular Biology-Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid Assay: 99% min. Assay Percent Range: (as Fatty Alcohol Sulfate): ≥99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Formula Weight: 288.38g/mol Synonym: SDS

Thermo Scientific™ POROS™ MabCapture™ A/G Select

MabCapture A/G Select allows antibody purification with high dynamic binding performance at low linear flow rates and maintains superior binding capacity at high linear flow rates

R&D Systems Sialyltransferase Activity Kit

A simple, nonradioactive high throughput-compatible format for assaying the enzyme activity of all sialyltransferases regardless of different acceptor substrates

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ Pierce™ DNase I

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Thermo Scientific™ Pierce™ D-Galactose Agarose

Bind and affinity-purify lectins, galactosidases and other galactose-binding molecules using this beaded agarose resin with immobilized thio-alpha-D-galactose.

Thermo Scientific Pierce™ Magnetic qIP Protein Interaction Kit, Tluc and HA Tags

Pull down (co-IP) and measure interactions between HA-tagged and Tluc-tagged protein pairs expressed in mammalian cells with this kit.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

EMD Millipore PureProteome™ Nickel Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Offer yields comparable with other magnetic purification systems while offering high purities

R&D Systems AEBSF, CF

A general, irreversible serine protease inhibitor

R&D Systems Proteome Purify™ 2 Human Serum Protein Immunodepletion Resin

A single-use resin that depletes serum or plasma of albumin and IgG to improve visualization of target proteins

Thermo Scientific™ Pierce™ c-Myc Tag IP/Co-IP Kit

Uses high-affinity antibodies to efficiently isolate c-Myc-tagged proteins and interactors

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ anti-HA Epitope Tag Clone: 2-2.2.14

HA Epitope Tag Monoclonal antibody 26181 from Thermo Scientific™ specifically detects HA Epitope Tag in Tag samples, and it is validated for Immunoprecipitation

Thermo Scientific™ MagnaBind™ Magnetic Beads and Magnets

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

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