Protein Extraction and Purification

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins

Acrylamide and agarose beads and columns of specially-modified, pH-stabilized avidin; the low-cost alternative to streptavidin biotin-binding supports.

Thermo Scientific™ Pierce™ Protein A/G Agarose and Kits

Affinity purify antibodies (IgG) with versatile, recombinant Protein A/G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Ubiquitin Enrichment Kit

Purify (enrich) poly-ubiquitinated proteins from cell and tissue lysates for post-translational modification studies using this affinity-resin, spin-column kit.

Thermo Scientific™ MagnaBind™ Magnetic Beads and Magnets

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ Protein L Agarose and Kits

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ NP-40 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent

Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent.

Thermo Scientific™ Pierce™ High-Capacity Protein A Agarose and Kits

Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ DNase I

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Thermo Scientific™ Pierce™ CHAPS

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ Pierce™ PES Protein Concentrators

Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific 1-Step Human High-Yield In Vitro Expression Kits

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

Thermo Scientific™ Pierce™ Protein G Agarose and Kits

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Thermo Scientific™ Pierce™ n-Dodecyl-β-Maltoside

Water-soluble, non-denaturing and nonionic detergent especially useful for solubilizing hydrophobic membrane proteins to preserve their activity.

Thermo Scientific™ Pierce™ Boronic Acid Resin

Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific Pierce™ Anti-c-Myc Agarose

Purify recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates with this immunoprecipitation resin.

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Tissue

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ Pierce™ IgG Plus Orientation Kits

Bind and covalently immobilize antibodies to create a reusable affinity purification column with high binding capacity with Protein A or G agarose columns and DSS crosslinker.

Thermo Scientific™ Protease Inhibitor Cocktails Available on GSA/VA Contract for Federal Government customers only.

Protect your sample from proteolytic degradation during cell lysis with these broad-spectrum protease inhibitor cocktails.

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ NHS-Activated Agarose

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Thermo Scientific™ Pierce™ CDI-Activated Agarose

Covalently immobilize N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions using carbonyldiimidazole-activated resin.

Thermo Scientific™ Y-PER™ Yeast Protein Extraction Reagent

Release functionally active solubilized proteins with the first commercially available yeast lysis reagent to use a mild detergent lysis procedure.

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