Protein Extraction and Purification

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

Thermo Scientific™ Pierce™ Protein G Agarose and Kits

Affinity purify antibodies (IgG) with immobilized recombinant Protein G agarose offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ n-Dodecyl-β-Maltoside

Water-soluble, non-denaturing and nonionic detergent especially useful for solubilizing hydrophobic membrane proteins to preserve their activity.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ GlycoLink™ Immobilization Kits and Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Thermo Scientific™ Pierce™ NHS-Activated Agarose

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Thermo Scientific™ Pierce™ High-Capacity Protein A Agarose and Kits

Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ MagnaBind™ Magnetic Beads and Magnets

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

Thermo Scientific™ Pierce™ IgG Plus Orientation Kits

Bind and covalently immobilize antibodies to create a reusable affinity purification column with high binding capacity with Protein A or G agarose columns and DSS crosslinker.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Thermo Scientific™ GlycoLink™ IP Kit

Immobilize polyclonal antibodies or other glycoproteins onto agarose beads for immunoprecipitation or pull-down assays via periodate-oxidized sugars.

Thermo Scientific™ Pierce™ Protein A Magnetic Beads

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ HiPPR Detergent Removal Resin and Kits

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

Thermo Scientific™ POROS™ MabCapture™ A/G Select

MabCapture A/G Select allows antibody purification with high dynamic binding performance at low linear flow rates and maintains superior binding capacity at high linear flow rates

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Thermo Scientific™ Pierce™ DNase I

Cleave and degrade unwanted single- and double-stranded DNA

Thermo Scientific™ Pierce™ Protein AG Magnetic Beads

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Protein L Agarose and Kits

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Thermo Scientific™ Pierce™ Direct IP Kit

Immobilize IP antibodies (any species or class) on agarose beads without Protein A/G to immunoprecipitate antigens without antibody interference.

Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit

Easily isolate and culture highly viable functional primary cardiomyocytes

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

  spinner